Method for preparing ginseng and larix olgensis extract and application of ginseng and larix olgensis extract to aspect of cosmetics
A technology of extract and larch, applied in the direction of cosmetics, cosmetic preparations, applications, etc., can solve the problem of less application and achieve the effect of improving anti-aging
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Embodiment 1
[0089] Example 1 Preparation method of Changbai Mountain red ginseng extract rich in AFG: crush 100g of red ginseng, pass through a 60-mesh sieve, stir overnight with 1000ml of high-concentration ethanol at 4°C, centrifuge, filter, remove the supernatant, and obtain a precipitate , then dissolved in water, filtered, the filtrate was ethanol precipitated, ethanol aqueous solution was reduced pressure to recover ethanol, centrifuged to remove the precipitate, the supernatant was passed through cation exchange resin, eluted with 0.1% citric acid aqueous solution, and the eluate rich in AFG was collected and passed through 400 Da hollow fiber filter membrane, take the cut-off part of the filter membrane, and freeze-dry to obtain 1.5 g of AFG-rich red ginseng extract. Use HPLC-ELSD method to detect the AFG content in the extract 85% AFG measurement spectrum sees attached figure 1 And attached figure 2 .
Embodiment 2
[0090] Example 2 The preparation method of the Changbai Mountain red ginseng extract rich in AFG
[0091]Take 100g of red ginseng and grind it, pass it through a 60-mesh sieve, stir overnight with 1000ml of high-concentration ethanol at 4°C, centrifuge, filter, remove the supernatant to obtain a precipitate, then dissolve it in water, filter, the filtrate ethanol precipitates, and decompress the ethanol water solution Recover ethanol, centrifuge to remove the precipitate, pass the supernatant through a cation exchange resin, elute with 0.1% acetic acid aqueous solution, collect the eluate from each part, combine the eluate rich in AFG components, pass through a 400Da hollow fiber filter membrane, and take the filter membrane to intercept part, freeze-dried to obtain 1.4g of red ginseng extract rich in AFG, and the AFG content in the extract was detected by HPLC-ELSD method to be 81%. figure 1 And attached figure 2 .
Embodiment 3
[0092] Example 3 Referring to the method of patent 201319302501.0, in short, take 1 kg of the root of artificially planted Changbai larch, wash it, remove the root bark, slice it, and crush it into sawdust; enzymatically hydrolyze the material, extract it by heating, concentrate it, sink it in water, remove the rosin precipitation, and extract it. Concentration, 135 g of crude crystals of the concentrated solution, repeated recrystallization, and drying to obtain 120 g of larch extract with a purity of 90% dihydroquercetin.
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