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UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules

A technology of Xuefu Zhuyu Decoction and fingerprints, which is applied in the field of UPLC fingerprint detection of Xuefu Zhuyu Decoction and capsules, can solve the problems of inability to comprehensively and scientifically evaluate the quality of Xuefu Zhuyu Decoction and capsules, and achieve reproducibility Good stability and stability, broad application prospects, high precision effect

Active Publication Date: 2015-12-23
TIANJIN HONGRENTANG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the prior art cannot comprehensively and scientifically evaluate the quality of Xuefu Zhuyu Decoction and capsules, the present invention proposes a UPLC fingerprint detection method for Xuefu Zhuyu Decoction and capsules

Method used

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  • UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules
  • UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules
  • UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] Concrete preparation method is as follows:

[0024] Xuefu Zhuyu Decoction: 9 grams of angelica, 9 grams of raw land, 12 grams of peach kernel, 6 grams of citrus aurantii, 9 grams of Achyranthes bidentata, 6 grams of red peony, 3 grams of Bupleurum, 6 grams of licorice, 4.5 grams of Chuanxiong, 4.5 grams of bellflower, 9 grams of safflower, soaked in 8 times the amount of distilled water for 1 hour, heated and refluxed for 2 hours, poured out the extract after cooling; added 8 times the amount of distilled water and heated and refluxed for 2 hours; combined the two extracts, concentrated under reduced pressure to obtain Extract; the extract is vacuum-dried at 40°C to obtain the dry extract of Xuefu Zhuyu Tang, crushed.

[0025] Xuefu Zhuyu Capsules: 81 grams of angelica, 81 grams of raw land, 108 grams of peach kernel, 54 grams of Citrus aurantii, 81 grams of Achyranthes bidentata, 54 grams of red peony, 27 grams of Bupleurum, 27 grams of licorice, 40 grams of Chuanxiong...

Embodiment 1

[0026] Example 1: Establishment of the Fingerprint Determination Method of Xuefu Zhuyu Decoction

[0027] 1.1 Instrument

[0028] ACQUITY ultra-high performance liquid chromatography (Waters, USA, including WatersACQUITY binary pump, DAD detector, automatic sampler, online degasser, column thermostat, Empower chromatography workstation); ultrapure water instrument (Milli-Q, American Millipore company); Vortex-5 vortex oscillator (Haimen Qilin Bell Instrument Manufacturing Co., Ltd.); J25 low-temperature high-speed centrifuge (American ThermoForma company); one hundred thousandth balance (METTLERTOLEDOXS205, Swiss METTLER company); One-fifth balance (METTLERTOLEDOAL204, Swiss METTLER company); SCIENTZSB25-12DTN ultrasonic cleaning machine (Ningbo Xinzhi Biotechnology Co., Ltd.); JSP-200 pulverizer (Zhejiang Yongkang Jinsui Machinery Manufacturing Factory).

[0029] 1.2 Reagents and Reagents

[0030] Sample: Xuefu Zhuyu Capsules: Tianjin Hongrentang Pharmaceutical Co., Ltd., b...

Embodiment 2

[0069] Example 2: Establishment of Fingerprint of Xuefu Zhuyu Decoction

[0070] 2.1 Preparation of the test solution

[0071] Take 3 different batches of Xuefu Zhuyu Decoction powder, take about 0.5g, weigh it accurately, put it in a 25mL brown measuring bottle, add an appropriate amount of 50% methanol aqueous solution, ultrasonicate for 20min, take it out, let it cool, add 50% methanol aqueous solution Dilute to the mark, shake well, and that's it.

[0072] 2.2 Chromatographic conditions:

[0073] Chromatographic column: ACQUITYUPLC? BEHC18 (2.1×100mm, 1.7μm); acetonitrile is used as mobile phase A, and 0.1% formic acid aqueous solution is used as mobile phase B. The volume ratio is that mobile phase A accounts for 3-97%, and mobile phase B Accounting for 97% to 3%, carry out gradient elution; the specific elution volume concentration preparation ratio is:

[0074] 0-8min, the volume ratio of mobile phase A is 3-8%, and the volume ratio of mobile phase B is 97%-92%;

[...

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Abstract

The invention discloses a UPLC fingerprint spectrum detection method of xuefu zhuyu decoction and capsules, and belongs to the field of column chromatography. According to the detection method, ultra-high performance liquid chromatography analysis is conducted on contents of xuefu zhuyu powder or capsules and a reference substance solution. Chromatographic conditions are that gradient elution is conducted by taking acetonitrile as a mobile phase A and taking a 0.1% formic acid aqueous solution as a mobile phase B, the detection wavelength is 280 nm, the column temperature ranges from 30 DEG C to 50 DEG C, the flow velocity ranges from 0.3 mL.min<-1> to 0.5 mL.min<-1>, and the injection volume is 2 microlitres. Fingerprint spectrum information of the xuefu zhuyu decoction and capsules is analyzed, 30 common peaks are totally obtained, the relative retention time and relative peak area of the common peaks are calculated by taking rhoifolin as a reference peak, and 16 common peaks are identified through a standard substance comparison method. The UPLC fingerprint spectrum detection method of the xuefu zhuyu decoction and capsules is comprehensive, rapid and reliable, fingerprint spectrum information of multiple components can be comprehensively obtained by means of the method, and therefore a reference basis is provided for quality control of the xuefu zhuyu decoction and capsules.

Description

technical field [0001] The invention belongs to the field of column chromatography, in particular to a UPLC fingerprint detection method for Xuefu Zhuyu Decoction and capsules. Background technique [0002] Fingerprint is a method to characterize the quality of medicines based on the research on the chemical components of traditional Chinese medicines, and systematically study the complex system of traditional Chinese medicines through a variety of analytical techniques. The fingerprint research of traditional Chinese medicine carries out similarity and correlation analysis from various aspects such as the production, rough processing, intermediates and finished products of medicinal materials, and finds quality variations and defects, so as to comprehensively and specifically control the quality of traditional Chinese medicine, especially suitable for traditional Chinese medicine and compound prescriptions Quality control studies of complex systems of formulations. Traditi...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 王跃飞朱晓晶杨龙杨欣莹江振作张蕾杨静
Owner TIANJIN HONGRENTANG PHARM CO LTD
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