A method for identifying soybean resistance to soybean blight and its application in breeding

A technology for blight resistance and Phytophthora sojae, applied in the directions of application, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of F2 population loss of heterozygous disease-resistant plants, and achieve the goal of avoiding population loss and improving efficiency. High controllability and small footprint

Inactive Publication Date: 2017-08-25
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to improve the identification efficiency of soybean resistance to soybean blight, and to avoid the defect that traditional identification methods will cause the loss of F2 population of heterozygous disease-resistant plants, and to provide a new method for identifying soybean resistance to soybean blight and its application in soybean breeding

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  • A method for identifying soybean resistance to soybean blight and its application in breeding
  • A method for identifying soybean resistance to soybean blight and its application in breeding
  • A method for identifying soybean resistance to soybean blight and its application in breeding

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Embodiment 1

[0035] Embodiment 1 A kind of method for identification soybean to soybean blight resistance

[0036] (1) Preparation of Phytophthora soybean inoculum

[0037] Phytophthora soybean strain ( 52696 TM ) were stored on the CA medium, and during the experiment, the bacterial strain was transferred to a petri dish containing 15ml of 1.5% agar-based CA medium, and cultivated in the dark at 25°C for 7-10 days, and the relative humidity of the culture environment was 90-100%. Inoculation of susceptible variety Williams (this variety is disclosed in "Genetic Analysis of Soybean Plant Introductionswith Resistance to Phytophthora sojae, Gordon SG, et al. Phytopathology, 2007Jan; 97 (1): 106-12", is a conventional soybean susceptible variety ) seedlings to recover the virulence, isolate and purify the pathogenic bacteria after the plants become ill, and use them for inoculation. One week before the inoculation, the bacterial strain was transferred into a petri dish containing 15 ml of...

Embodiment 2

[0045] Embodiment 2 A kind of method for identification soybean to soybean blight resistance

[0046] (1) Preparation of Phytophthora soybean inoculum

[0047] Phytophthora sojae strains are preserved on the carrot juice agar (CA) medium. During the experiment, the strains are transferred to the CA medium petri dish containing 15ml of 1.5% agar, cultivated in the dark at 22°C for 7-10 days, and inoculated with the susceptible variety Williams The seedlings recovered their virulence, and the pathogenic bacteria were isolated and purified after the onset of the plants, and used for inoculation. One week before inoculation, the strains were transferred to a petri dish containing 15 ml of CA medium containing 1.5% agar, and cultivated in the dark at 27°C for 7-10 days. When inoculating, use a Joyoung Soymilk machine to crush the culture medium containing mycelium to prepare inoculum, dispense it into 5ml syringes, and prepare for inoculation.

[0048] (2) Inoculate the soybean p...

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Abstract

The invention provides a method for identifying soybean resistance to soybean blight and its application in breeding. The method comprises the steps of: inoculating the Phytophthora sojae strain on the CA medium, the V8 medium or the Lima bean medium, culturing in the dark to rejuvenate the strain, and then crushing the medium rich in the bacterial cells to obtain the inoculum; (2) inoculating soybean plant material: the inoculum obtained in step (1) is contained in a detection container, and the green stalked leaf just flattened of the soybean plant to be inspected is selected, and the petiole is inserted into the inoculum in the detection container; ( 3) Dark cultivation: The detection container inoculated with the soybean material in step (2) is placed in a humid closed space and the result is judged after dark cultivation. The identification method of the invention is easy to operate, low in cost, saves time, and can carry out repeated experiments without losing the experimental materials to be identified, and avoids the disadvantage that the traditional method will cause mass loss of experimental materials.

Description

technical field [0001] The invention belongs to the technical field of disease-resistant breeding, and in particular relates to a method for identifying soybean blight resistance and its application in soybean breeding. Background technique [0002] Soybean blight caused by Phytophthora sojae Kaufmann & Gerdemann is a devastating soil-borne disease in soybean production. The disease has occurred in many provinces of our country. Phytophthora sojae can infect soybeans in all growth stages of soybeans, causing root rot, stem rot, plant dwarfing, withering and death. Generally, the yield of fields is reduced by 10%-30%, and in severe fields it can reach 60%-90%. even lead to miscarriage. The current worldwide economic loss due to soybean blight is approximately US$ 1 billion per year (Tyler, 2007). [0003] At present, researchers generally identify the resistance of soybean to soybean blight through hypocotyl wound inoculation. The hypocotyl inoculation method can only be ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18A01H3/00A01H3/04
Inventor 朱振东李银萍孙素丽钟超王晓鸣
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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