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Cordyceps militaris liquid strain stabilizing technology

A liquid strain, Cordyceps militaris technology, applied in horticulture, application, botanical equipment and methods, etc., to achieve the effect of stable content

Inactive Publication Date: 2015-12-23
EASTERN LIAONING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also many Cordyceps workers who have done a lot of research and discussion on how to increase the content of cordycepin in production, but so far there is no standard for a stable content, and they are always hovering between high or low or even presence or absence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] S1. Take 60 parts of corn cobs, 30 parts of sawdust, 11 parts of glucose, 3 parts of peptone, 7 parts of agar, 90 parts of pine needle nutrient solution, 0.3 parts of magnesium sulfate, 0.3 parts of potassium dihydrogen phosphate, 0.2 parts of manganese amino acid chelate, and a Mix 0.2 parts of zinc sulfate water and put it into a triangular flask, sterilize, cool, and place it on an inclined plane, inoculate the mycelium of Cordyceps sinensis, and culture it in a 20°C incubator in the dark for 3 days, then cool it down to 20°C and cultivate it for 4 days. day, then 24 hours under 18 natural light;

[0019] S2. Weigh maltose 20g / L, peptone 30g / L, MnSO4 powder 0.1g / L, CuSO 4 Powder 0.02g / L, ZnSO4 powder 0.1g / L, NiSO4 powder 0.2g / L, (NH4)6Mo7O24.4H2O powder 0.2g / L, after mixing, sterilize, the volume ratio is 50% of this liquid culture medium amount, inoculate the mycelium obtained in step S1, and culture it in a liquid state on a rotary shaker at 20° C. and 180 r / min f...

Embodiment 2

[0023] S1. Take 110 parts of corn cobs, 40 parts of wood chips, 15 parts of glucose, 5 parts of peptone, 13 parts of agar, 196 parts of pine needle nutrient solution, 0.7 parts of magnesium sulfate, 0.7 parts of potassium dihydrogen phosphate, 0.3 parts of manganese amino acid chelate, and a Mix 0.3 parts of zinc sulfate water and put it into a triangular flask, sterilize, cool, and place it on an inclined plane, inoculate the mycelium of Cordyceps sinensis, and culture it in a 25°C incubator in the dark for 4 days, then cool it down to 20°C and cultivate it for 5 days. day, and then 24 hours under natural light at 22°C;

[0024] S2. Weigh maltose 24g / L, peptone 34g / L, MnSO4 powder 0.4g / L, CuSO 4 Powder 0.1g / L, ZnSO4 powder 0.2g / L, NiSO4 powder 0.4g / L, (NH4)6Mo7O24.4H2O powder 0.4g / L, after mixing, sterilize, be 60% of this liquid culture medium by volume ratio amount, inoculate the mycelium obtained in step S1, and culture it in a liquid state on a rotary shaker at 24° C. an...

Embodiment 3

[0028] S1. Take 85 parts of corncobs, 35 parts of sawdust, 13 parts of glucose, 4 parts of peptone, 10 parts of agar, 143 parts of pine needle nutrient solution, 0.5 parts of magnesium sulfate, 0.5 parts of potassium dihydrogen phosphate, 0.25 parts of manganese amino acid chelate, and a Mix 0.25 parts of zinc sulfate in water and put it into a triangular flask, sterilize, cool, and place it on an inclined plane, inoculate the mycelium of Cordyceps sinensis, and culture it in the dark for 3.5 days in an incubator at 22.5°C, then cool it down to 20°C and cultivate it for 4.5 days. day, and then 24 hours under natural light at 20°C;

[0029] S2. Weigh maltose 22g / L, peptone 32g / L, MnSO4 powder 0.25g / L, CuSO 4 Powder 0.06g / L, ZnSO4 powder 0.15g / L, NiSO4 powder 0.3g / L, (NH4)6Mo7O24.4H2O powder 0.3g / L, after mixing, sterilize, be 55% of this liquid culture medium by volume ratio amount, inoculate the mycelium obtained in step S1, and culture it in a liquid state on a rotary shaker...

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Abstract

The invention discloses a cordyceps militaris liquid strain stabilizing technology. The technology comprises the step of cultivating a plant expanding culture medium obtained by mixing 60-110 parts of corncobs, 30-40 parts of saw dust, 11-15 parts of glucose, 3-5 parts of peptone, 7-13 parts of agar, 90-196 parts of a pine needle nutrient solution, 0.3-0.7 part of magnesium sulfate, 0.3-0.7 part of monopotassium phosphate, 0.2-0.3 part of amino acid manganese chelate and 0.2-0.3 part of zinc sulphate monohydrate, the step of cultivating a solid culture medium obtained by mixing maltose, peptone, MnSO4 powder, CuSO4 powder, ZnSO4 powder, NiSO4 powder and (NH4)6Mo7O24.4H2O powder and the step of cultivating a culture medium obtained by mixing potato pulp, sweet potato vines, a yeast extract, manganese methionine and selenium methionine. The content of the cordycepin in the obtained product is not lower than 0.2%, and the content is stable.

Description

technical field [0001] The invention relates to the field of cultivation of fungal fruit bodies, in particular to a technology for stabilizing liquid strains of Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps genus Ergotaceae of Ascomycetes Hypocreales. my country began to study artificial Cordyceps militaris cultivation technology from the 1980s, and has formed a conventional cultivation technology and entered the stage of industrialization. Cordyceps militaris is emerging as a new type of industry, but ordinary growers pay more attention to the cultivation yield, ignoring the importance of product function, and little research has been done on its active ingredients, especially the stable improvement of the content of cordycepin. In recent years, domestic and foreign medical experts and scholars have conducted in-depth research on cordycepin, and have reached a consensus on its impor...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 刘晓红
Owner EASTERN LIAONING UNIV
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