Simultaneous screening and detection method of plurality of types of veterinary drug residues in solid animal-derived foods

Abstract

The invention relates to a simultaneous screening and detection method of a plurality of types of veterinary drug residues in solid animal-derived foods. The simultaneous screening and detection method comprises the following steps: after taking and crushing a sample to be detected, adding an acetonitrile acetate solution and a Na2EDTA buffering solution into the sample to be detected; meanwhile, adding anhydrous sodium sulfate and sodium chloride, and homogenizing and extracting; adding an adsorbent containing C18 and NH2 into extracting-obtained liquid supernatant to carry out purification; then determining by using high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry. The screening method provided by the invention can be used for simultaneously screening and detecting the plurality of types of veterinary drug residues in the animal-derived foods, has the characteristics of rapidness, simplicity and convenience, flexibility, accuracy and the like, can be used for carrying out an accurate qualitative analysis on target veterinary drugs in the sample, and also can be used for carrying out quantitative detection. The method is applicable to rapid detection of the plurality of types of veterinary drug residues in batch samples. Aiming at the solid animal-derived foods, the impact on the target veterinary drugs by sample substrates is reduced, and the extraction effect is good and the recycling rate is high.

Description

Technical field: [0001] The invention relates to a method for simultaneous screening and detection of multiple types of veterinary drug residues in solid animal-derived food, and belongs to the technical field of food analysis. Background technique: [0002] Food safety has attracted widespread attention from the whole society, and veterinary drug residues are one of the main factors affecting the safety of animal-derived food. With the development of my country's large-scale breeding industry, various antibiotics and other drugs are not only used to treat animal diseases, but also added to animal feed or drinking water for non-therapeutic purposes to promote animal growth and improve feed conversion rate , Improve product quality. Excessive and illegal use of these drugs will inevitably lead to veterinary drug residues in animal-derived foods, endangering the health of consumers, and long-term consumption can easily induce bacterial drug resistance. Therefore, it is impera...

AI Technical Summary

Problems solved by technology

The methods of ELISA, GC and HPLC have low sensitivity, poor selectivity and specificity; although GC-MS, LC-MS and LC-MS / MS have high sensitivity and specificity, it is difficult to fully elucidate the compound Structural cleavage information, qualitative accuracy is still lacking, prone to false positive results, and the number of compounds that can be detected simultaneously is limited

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