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Construction Method and Application of Liquid Chromatographic Fingerprint of Shanxi Mature Vinegar

A fingerprint and construction method technology, which is applied to the fingerprint construction method of Shanxi mature vinegar and its application in quality control, can solve the problems of narrow detection range, cumbersome processing process, and inability to fully reflect the internal quality of Shanxi mature vinegar. To achieve the effect of simple pre-processing and short time consumption

Active Publication Date: 2017-04-26
阳泉市裕盛源农产品开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing studies have either focused on its aroma, that is, volatile components, or on its organic acid components. Not only is the sample pretreatment process cumbersome, but more importantly, the detection range is narrow, which cannot fully reflect the internal quality of Shanxi mature vinegar.

Method used

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  • Construction Method and Application of Liquid Chromatographic Fingerprint of Shanxi Mature Vinegar
  • Construction Method and Application of Liquid Chromatographic Fingerprint of Shanxi Mature Vinegar
  • Construction Method and Application of Liquid Chromatographic Fingerprint of Shanxi Mature Vinegar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 1 ml of Shanxi aged vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the solution to be tested. The hydrophilic chromatographic column Synrgi 4u Hydro-RP 80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0034] Flow rate: 0.8 mL / min; detection wavelength: 254 nm; column temperature: 26 °C; injection volume: 5 μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% formic acid Aqueous solution, elution procedure: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

Embodiment 2

[0036] Take 1 ml of Shanxi aged vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the solution to be tested. The hydrophilic column Synrgi 4u Hydro-RP 80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0037] Flow rate: 0.5mL / min; detection wavelength: 260nm; column temperature: 26°C; injection volume: 5μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% formic acid Aqueous solution, elution procedure: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

Embodiment 3

[0039] Take 1 ml of Shanxi aged vinegar sample (sample No. 1), filter it with a 0.22 μm microporous membrane, and take the subsequent filtrate to obtain the solution to be tested. The hydrophilic column Synrgi 4u Hydro-RP 80A (250×4.60mm) was used as the stationary phase, and the test was carried out according to the following liquid phase conditions:

[0040] Flow rate: 0.8 mL / min; detection wavelength: 240 nm; column temperature: 30 °C; injection volume: 5 μL; linear gradient elution; mobile phase: mobile phase A is acetonitrile, mobile phase B is 0.1% phosphoric acid Aqueous solution, elution procedure: 0~5min, 0% phase A; 5~21min, 0%~6.4% phase A; 21~59min, 6.4%~30% phase A; 59~62min, 30%~0% phase A , 62 ~ 65min, 0% A phase.

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Abstract

The present invention discloses a construction method and applications of a Shanxi mature vinegar liquid-phase fingerprint, and relates to the technical field of fingerprint construction. The construction method comprises: (1) taking 1 mL of Shanxi mature vinegar, and filtering by using a 0.22 [mu]m micro-pore filtration membrane for spare; and (2) sucking the filtered Shanxi mature vinegar, and determining through a high-pressure liquid chromatograph to obtain the chemical fingerprint. According to the present invention, the sample pre-treatment is simple and easy, the chemical components and the quality characteristics of the Shanxi mature vinegar can be completely reflected, the Shanxi mature vinegar liquid-phase fingerprint can be used for the quality control of the Shanxi mature vinegar and can further provide technical support of the protection of the Shanxi mature vinegar having the origin production region and the geographical indication.

Description

technical field [0001] The invention relates to the construction of a fingerprint, in particular to a method for constructing a fingerprint of Shanxi aged mature vinegar based on liquid chromatography technology and its application in quality control. Background technique [0002] Shanxi old mature vinegar is a characteristic industry in Shanxi Province with a long history. With the development of various analytical methods and instruments, the systematic research on the chemical composition of vinegar has gradually deepened. It has been reported that vinegar contains sugars, amino acids, acids, alcohols, aldehydes, esters, polyphenols, etc. Element. [0003] In recent years, the content of ligustrazine and total flavonoids has been increased in the quality standard of Shanxi old mature vinegar. However, the composition of Shanxi old mature vinegar is very complex, and it is difficult to ensure the uniform and stable quality of only controlling the content of ligustrazine ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 李震宇范玛莉邢婕张丽增秦雪梅
Owner 阳泉市裕盛源农产品开发有限公司
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