Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device

A technology of hyaluronic acid and selection device, applied in the fields of biochemical cleaning device, enzymology/microbiology device, bioreactor/fermenter combination, etc. It can save the operator's time, save time and improve work efficiency.

Inactive Publication Date: 2015-12-16
BRED LIFE SCI TECH
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Most of the petri dishes used to select sperm are ordinary cell culture dishes (Petridish). Put the processed semen into ordinary cell culture dishes and observe the motility of sperm under a microscope. Experience judges and selects mature sperm that are considered to be of high quality. As we all know, sperm with strong motility is not necessarily mature sperm. This selection process is greatly affected by the operator's personal experience and subjectivity, and has obvious randomness. Ensure that the active sperm selected are mature motile sperm

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device
  • Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device
  • Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A single sperm selection device coated with hyaluronic acid and its salts or derivatives, comprising a dish body 2 and an adapted dish cover 1, preferably made of transparent hard plastic; the size of the culture dish body 2 is: The diameter is 50 mm, and the height is 8 mm. The center of the inner bottom of the dish body 2 is integrally formed with an upward annular protrusion to form the small groove 21. The height of the small groove 21 is higher than that of the coated solid-phase transparent The height of the hyaluronic acid, the small groove 21 is coated with solid-phase hyaluronic acid that can bind mature sperm. The small groove 21 can also be arranged at other suitable positions on the inner bottom of the dish body 2, and the small groove 21 can be coated with solid-phase hyaluronic acid and its salt or derivatives that can also bind mature sperm heads.

[0033] The size of the small groove 21 is preferably: a diameter of 9 mm and a height of 0.6 mm. According ...

Embodiment 2

[0045] The single sperm selection device coated with hyaluronic acid and its salts or derivatives in Example 2 is the same as that in Example 1, the only difference is that the method used to select mature sperm is different. When the operator selects mature single sperm, press Follow these steps:

[0046] 1) taking the single sperm selection device out of the refrigerator where it was placed, and equilibrating to room temperature;

[0047] 2) Separation and washing of sperm by the density gradient method, and other methods of separation and washing of sperm in the prior art can also be adopted as required; the number of sperm is adjusted to 0.5×10 with a culture solution containing 4 mg / ml human albumin. 6 sperm suspension per ml to improve the recognition accuracy of sperm specifically bound by hyaluronic acid and its salts or derivatives; it can also be adjusted to a sperm count of 0.2 with a culture medium containing 3-5 mg / ml human albumin ~1×10 6 Sperm suspension per m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Heightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives. The device comprises a small groove (21) integrally formed in the bottom in a petri dish body (2); the small groove (21) is coated with hyaluronic acid, and hyaluronates or hyaluronic acid derivatives capable of being combined with mature sperms, and is higher than the coated hyaluronates or hyaluronic acid derivatives; a plurality of small lugs (22) which allow the petri dish body (2) to be taken conveniently and avoid contact of a petri dish opening of the petri dish body (2) are distributed on the outer side of the petri dish body (2); a convex step (23) for preventing the outer bottom of the petri dish body (2) from being worn is arranged at the outer bottom of the petri dish body (2); an angle formed between the highest part of the edge of the petri dish body (2) and the bottom of the small groove (21) is 5-23 degrees. The invention further discloses an application method of the device. The device is easy to use and convenient to operate; through the adoption of the device, the mature sperms can be selected conveniently without destroying morphology, structure and vitality of the sperms.

Description

technical field [0001] The invention relates to the field of sperm selection in vitro, and relates to a single sperm selection device coated with hyaluronic acid and its salt or derivatives and its application. Background technique [0002] Most of the petri dishes used to select sperm are ordinary cell culture dishes (Petridish). Put the processed semen into ordinary cell culture dishes and observe the motility of sperm under a microscope. Experience judges and selects mature sperm that are considered to be of high quality. As we all know, sperm with strong motility is not necessarily mature sperm. This selection process is greatly affected by the operator's personal experience and subjectivity, and has obvious randomness. Make sure that the selected motile sperm are mature motile sperm. [0003] Hyaluronic acid is the main component of the mucus matrix of eggs and zona pellucida, and hyaluronidase is contained in the acrosome and plasma membrane of sperm. When sperm pene...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/22
CPCC12M47/04C12N5/061C12N2509/00
Inventor 胡家纯陶思恩程浩邓少君杨红芳何水生
Owner BRED LIFE SCI TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com