Method for preparing sericin from tussah silk degumming industrial wastewater

Abstract

The present invention relates to a method for preparing sericin from tussah silk degumming industrial wastewater. According to the present invention, tussah silk industrial wastewater is adopted as a raw material, the total content of the protein in the solution is detected by using a Folin-phenol colorimetry method, the color and the presence of the precipitate of the tussah silk industrial wastewater are observed, the pH value is measured, the sericin in the solution is subjected to crude extraction by using a salting out method, a large amount of salt ions in the protein solution are removed through dialysis operation, and the sericin in the solution is further extracted through an organic precipitation method to improve the protein purity in the solution so as to achieve the sericin preparation from the tussah silk production industrial wastewater; and with the method, the environmental pollution can be prevented, the sewage treatment problem is solved, and the extracted sericin can be used in the fields of cosmetics, food, fiber and textiles, medicine, biological materials and the like, and has important significance in the research of the modern new industry.

Description

technical field [0001] The invention relates to a method for preparing sericin protein from tussah silk degumming industrial wastewater, which uses a salting-out method to prepare high-purity sericin protein that can be used in the fields of medicine, cosmetics, food and the like. Background technique [0002] Tussah silk is one of the unique natural textile raw materials in my country, which is composed of silk fibroin and sericin. Among them, sericin on the periphery of silk fibroin accounts for 20%-30% of the total amount of silk. In silk, it wraps two monofilaments together to play an adhesive role. It has hygroscopicity, solubility, anticoagulation and antioxidation. And so on. Sericin is a sericin-like globulin with a relative molecular weight of 14,000-314,000. It is composed of 18 kinds of amino acids, mainly including serine, aspartic acid, glutamic acid and tyrosine. The secondary structure of sericin is mainly non-coil, and contains part of β conformation, almos...

AI Technical Summary

Problems solved by technology

④ Sericin can be used as an immobilized enzyme carrier, and the use of sericin membrane as an immobilized enzyme carrier can significantly improve the heat resistance, electroosmotic resistance, and enzyme activity stability of the enzyme, but the yield of the immobilized enzyme is low

However, sericin is high-concentration organic wastewater. If it is directly discharged into the river without treatment, it will consume a large amount of dissolved oxygen in the water, resulting in a serious excess of chemical oxygen demand in the water, destroying the original water quality, and polluting the local water body. , which not only causes environmental pollution, but also discards a large amount of sericin, which also causes a waste of resources

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