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Method of treating piggery methane fluid by utilizing composite photosynthetic bacteria

A treatment method and compound light technology, which is applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve rare research problems and achieve the effect of easy operation and wide application

Inactive Publication Date: 2015-12-09
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, nowadays, the research and application of photosynthetic bacteria on wastewater treatment are mostly focused on pure photosynthetic bacteria, such as patent number CN201210574326.6 "Using Photosynthetic Bacteria to Treat Food Processing Wastewater and Realize Recycling Method", the strain used is Rhodopseudomonas palustris Bacteria; Patent No. CN201210574326.6 "Using Photosynthetic Bacteria to Treat Food Processing Wastewater and Realize Recycling Method" uses Rhodopseudomonas palustris; Patent No. CN201210165608 "Using a Photosynthetic Bacteria to Treat Starch Wastewater and Realize Recycling "method" uses Rhodobacter sphaericus, and there are few studies on the use of multiple pure photosynthetic bacteria to treat wastewater

Method used

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  • Method of treating piggery methane fluid by utilizing composite photosynthetic bacteria
  • Method of treating piggery methane fluid by utilizing composite photosynthetic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Treatment of pig farm biogas slurry by composite photosynthetic bacteria

[0023] Step A: preparation of composite photosynthetic bacteria, budding rhodobacteria and capsulated rhodobacteria are cultivated according to the following steps:

[0024] (1) budding rhodobacteria and capsular rhodobacteria on the slant culture medium of purified culture are picked 1~2 rings respectively with inoculation loop, respectively inoculate in two Erlenmeyer flasks that the bacterium activation liquid culture medium of 100ml is housed in two bottles, Under the conditions of pH 7.0, light intensity 3000 lux, and temperature 30°C, static culture was carried out for 4 days to obtain one bottle of liquid culture solution containing activated budding rhodobacter and one bottle of liquid culture solution containing activated capsulated rhodobacter.

[0025] Wherein, the composition of the bacterial activation liquid medium is as follows: 10g / L peptone, 6g / L yeast extract powder, ...

Embodiment 2

[0030] Embodiment 2: Rhodobacter capsularis processing swine farm biogas slurry

[0031] This case is a comparative test of Case 1. Only Rhodobacter capsulata is used to treat pig farm biogas slurry, and other treatment steps are the same.

[0032] Step A: the preparation of photosynthetic bacteria, the capsulated rhodobacter is cultivated according to the following steps:

[0033] (1) Pick 1 to 2 loops of capsulated rhodobacteria on the slant medium of purified culture with an inoculation loop, inoculate into a Erlenmeyer flask with 100ml of bacterial activation liquid medium in a bottle, at pH7.0, light intensity Under the conditions of 3000 lux and temperature of 30° C., static culture was carried out for 4 days to obtain a bottle of liquid culture solution containing activated rhodobacter capsulata.

[0034] Wherein, the composition of the bacterial activation liquid medium is as follows: 10g / L peptone, 6g / L yeast extract powder, 1.2g / L glucose, the medium was sterilized ...

Embodiment 3

[0039] Example 3: Rhodobacter budding bacteria treatment of swine farm biogas slurry.

[0040] This case is a comparative test of Case 1 and Case 2. Only rhodobacter budding was used to treat pig farm biogas slurry, and other treatment steps were the same.

[0041] Step A: the preparation of photosynthetic bacteria, the budding rhodobacter is cultivated according to the following steps:

[0042] (1) Pick 1 to 2 rings of budding rhodobacteria on the slant medium of purified culture with an inoculation loop, inoculate into a Erlenmeyer flask with 100ml of bacterial activation liquid medium in a bottle, at pH7.0, light intensity Under the conditions of 3000 lux and a temperature of 30° C., static culture was carried out for 4 days to obtain a bottle of liquid culture solution containing activated bud-forming rhodobacteria.

[0043] Wherein, the composition of the bacterial activation liquid medium is as follows: 10g / L peptone, 6g / L yeast extract powder, 1.2g / L glucose, the medi...

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Abstract

The invention discloses a method of treating piggery methane fluid by utilizing composite photosynthetic bacteria, and relates to the direction of wastewater treatment and achieves the purpose of waste recycling. According to the method, the composite photosynthetic bacteria which contain budding bacteria and rhodobacter capsulatus are prepared; the piggery methane fluid is treated by utilizing the composite photosynthetic bacteria according to a dosage amount of (1 to 5) to (1 to 10) of the volume ratio of the composite photosynthetic bacteria to the methane fluid, and the processing temperature is 25 to 32 DEG C, and the illumination intensity is 2000 to 5000 lux. According to the method, methane fluid wastewater is treated by utilizing the synergistic effect of mixed bacteria, and compared with a method of treating the methane fluid by utilizing single bacterium, the removal efficiency of COD (chemical oxygen demand) is increased by more than 20%. According to the method, the synergistic effect of two types of photosynthetic bacteria is utilized, so that the growth rates of the two types of photosynthetic bacteria in the piggery methane fluid are increased, thereby increasing the utilization rate of the photosynthetic bacteria for organic matter, ammonia nitrogen, phosphorus and the like in the piggery methane fluid, overcoming the defect of poor treatment effect of the single bacterium on the methane fluid and increasing the treatment and purification efficiency of the composite bacteria for the methane fluid. Additionally, a large number of output bacteria can be comprehensively utilized directly, so that the purposes of removing pollutants and obtaining bacteria resources are achieved.

Description

technical field [0001] The invention relates to the field of waste water treatment, in particular to a method for treating biogas slurry from pig farms by using composite photosynthetic bacteria, which also achieves the purpose of recycling waste while treating the biogas slurry. Background technique [0002] There are many domestic treatment methods for pig farm wastewater. Among them, the process of using anaerobic process to treat pig farm wastewater and recover biogas slurry is relatively mature. However, the sewage treated by anaerobic process still has high pollutant content and cannot be discharged directly. , the pressure on the environment is still great. With the country's increasing emphasis on environmental protection and the strict implementation of sewage discharge standards, the treatment and discharge of biogas slurry from pig farms has become an important factor affecting the survival and development of pig farms. [0003] The use of photosynthetic bacteria...

Claims

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Application Information

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IPC IPC(8): C02F3/34
CPCY02E50/30
Inventor 杨春平文珊刘红玉罗乐周自力曾光明娄伟何慧军
Owner HUNAN UNIV
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