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Preparation method of hydrogel for cartilage repair and with tissue inductivity

A cartilage repair and hydrogel technology, applied in medical science, prosthesis, etc., can solve the problems of expensive growth factors, activity damage, short half-life, etc., to achieve growth and repair, strong resistance to damage, The effect of easy preparation

Inactive Publication Date: 2015-12-09
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, growth factors are expensive, have a short half-life, and are easily denatured. Dropping growth factors directly on the surface of the hydrogel or directly swelling the solution containing the factors with the hydrogel is prone to burst release, and may also cause toxicity and carcinogenicity due to local overdose
The problem of burst release and local excess toxicity can be solved by covalently immobilizing the factor in the group of the hydrogel component, but the activity of the factor will be destroyed by the covalent reaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A. Double Bonding of Gelatin

[0028] Gelatin was dissolved in phosphate buffered saline to obtain a solution with a gelatin mass concentration of 10%, then, methacrylic anhydride 0.8 times the mass of gelatin was added dropwise to the solution, and then stirred and reacted for 3 hours at 50°C; subsequently, The solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0029] B. Preparation of hydrogel

[0030] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 20%, and the mass concentration of the methacrylamide dopamine monomer is 4%. Then, the photoinitiator irgacure2959 is added to the mixed soluti...

Embodiment 2

[0034] A. Double Bonding of Gelatin

[0035] Dissolving the gelatin in a phosphate buffer solution to obtain a solution with a gelatin mass concentration of 10%, then adding glycidyl methacrylate 17.5 times the mass of the gelatin dropwise to the solution, and then stirring and reacting at 50°C for 3 hours; Subsequently, the solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0036] B. Preparation of hydrogel

[0037] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 15%, and the mass concentration of the methacrylamide monomer is 3%. , the photoinitiator irgacure127 is added to the mixed solution, the ...

Embodiment 3

[0041] A. Double Bonding of Gelatin

[0042]Dissolving the gelatin in a phosphate buffer solution to obtain a gelatin solution with a mass concentration of 10%, then adding glycidyl methacrylate 0.16 times the mass of the gelatin dropwise to the solution, and then stirring and reacting at 50°C for 3 hours; Subsequently, the solution was diluted 5 times, then dialyzed and dried to obtain double-bonded gelatin;

[0043] B. Preparation of hydrogel

[0044] Dissolving the methacrylamidated dopamine monomer in dimethyl sulfoxide to obtain a methacrylamidated dopamine monomer solution, and then co-dissolving the methacrylamidated dopamine monomer solution and the double bonded gelatin obtained in step A in A mixed solution is prepared in the phosphate buffer solution, the mass concentration of the double-bonded gelatin in the mixed solution is 20%, and the mass concentration of the methacrylamide monomer is 5%. , the photoinitiator irgacure184 is added to the mixed solution, the m...

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Abstract

The invention discloses a preparation method of hydrogel for cartilage repair and with tissue inductivity. The preparation method comprises the following main steps: A, polysaccharide or protein double bonding, B, hydrogel preparation, and C, adsorption of growth factors. According to the preparation method, double bonded natural polysaccharide / protein and methacrylamide dopamine which are completely degradable are used as the main components, and hydrogel adopting a double network structure can be formed through free radical polymerization of two materials of double bonded polysaccharide / protein and methacrylamide dopamine. Then hydrogel adsorbs biological activity growth factors through the dehydration and reswelling functions, and the growth factors are fixed into hydrogel throguh the dopamine component. The hydrogel obtained according to the preparation method is excellent in biological degradability and cell / tissue affinity, high in activity of the loaded growth factors and good in slow release performance, and can better induce cartilage regeneration and promote cartilage repair.

Description

technical field [0001] The invention relates to a method for preparing a tissue-inducing hydrogel for cartilage repair Background technique [0002] The structure of hydrogel is similar to the extracellular matrix of natural cartilage. It has been widely used in the research of cartilage defect repair and is expected to become a cartilage replacement material. However, the poor mechanical properties of hydrogels make it difficult to adapt to various mechanical environments in joints, which limits their application as cartilage replacement materials. The latest research has found that the double-network structure hydrogel has good mechanical properties, and can even make the mechanical properties of the hydrogel reach or exceed those of cartilage. The existing preparation method of the double network structure hydrogel is mainly to first polymerize a monomer network, then put it into the solution containing the second layer network monomer to swell and then polymerize the se...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/24A61L27/20A61L27/52A61L27/54C08F290/10C08F290/00
Inventor 鲁雄胥杰龙甘东林王振铭张晏宁
Owner SOUTHWEST JIAOTONG UNIV
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