A preparation of megakaryotic progenitor cells and its application and preparation method
A technology of megakaryotic progenitor cells and preparations, applied in the field of stem cell preparations, can solve problems such as prone to adverse reactions and unsatisfactory treatment effects
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Embodiment 1
[0079] Example 1 Preparation of megakaryotic progenitor cells
[0080] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.
[0081] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.
[0082] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 2mL human serum albumin, 1.5mL stem cell growth factor, 0.5mL interleukin 2 and 11mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.
[0083] This megakaryotic progenitor cell preparation includes:
[0084]
Embodiment 2
[0085] Example 2 Preparation of megakaryotic progenitor cells
[0086] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.
[0087] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.
[0088] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 3mL human serum albumin, 1mL stem cell growth factor, 1mL interleukin 2 and 10mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.
[0089] This megakaryotic progenitor cell preparation includes:
[0090]
[0091]
Embodiment 3
[0092] Example 3 Preparation of megakaryotic progenitor cells
[0093] 1. Take 0.5~1×10 8 The megakaryotic progenitor cells cultured for two weeks were transferred to a 50mL centrifuge tube and centrifuged at 300g for 5min.
[0094] 2. Discard the supernatant, add 40mL 10% glucose injection to the centrifuge tube to wash the cells, centrifuge at 200g for 5min, and wash twice.
[0095] 3. Discard the supernatant and resuspend 1×10 with 80mL 10% glucose injection 8 Megakaryotic progenitor cells, and add 4mL human serum albumin, 0.5mL stem cell growth factor, 1.5mL interleukin 2 and 9mL safflower injection. After mixing well, collect it in a 100mL infusion bag for later use.
[0096] This megakaryotic progenitor cell preparation includes:
[0097]
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