Production method of lactic acid bacteria fermented tied pig leg meat with the addition of zymolytic bone paste
The technology of lactic acid bacteria fermentation and production method is applied in the production field of lactic acid bacteria fermentation bound hoof, can solve the problems of single taste, short shelf life, low calcium content and the like, and achieves the effects of better flavor, convenient eating and high calcium content
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[0034] Preparation of starter:
[0035] Inoculate Lactobacillus bulgaricus in MRS medium, 32-40°C, natural pH value, static culture for 20-24h to the logarithmic growth phase, centrifuge at 4000r / min for 30min at 4°C to collect the sludge, and sterilize the physiological saline Wash 2 to 3 times to make 10 7 ~10 8 cfu / mL starter, set aside.
[0036] MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, dipotassium hydrogen phosphate 2g, glucose 20g, sodium acetate 5g, diamine citrate 2g, Tween 801mL, magnesium sulfate 0.58g, manganese sulfate 0.25g, distilled water 1000mL , pH6.2~6.4.
Embodiment 1
[0038] A method for making lactic acid bacteria fermented hooves added with enzymatic bone paste, comprising the following steps:
[0039] (1) Raw material preparation
[0040] A. Prepare pigskin: clean the raw pigskin, remove the fat and hair on the skin, remove abnormal skins such as seals and belly milk skin, soak in 2% sodium lactate solution for 12 hours, take it out with 0.8% H 2 o 2 Solution bleaching, bleaching temperature 35 ° C, bleaching time 40 minutes, then cut the pigskin into pieces, boiled in water for 10 minutes, drained and cooled for later use;
[0041] B. Prepare raw meat: thaw and wash the frozen hoof meat, remove large pieces of fat, wash with running water, drain, and mince to get 0.3-0.5cm granular meat filling;
[0042] C. Prepare enzymatic bone paste:
[0043] Select fresh pig bones, remove the excess meat and other sundries on the bones, and clean them; put the cleaned pig bones in a high-pressure steam sterilizer, and cook them at 115°C for 50 mi...
Embodiment 2
[0055] A method for making lactic acid bacteria fermented hooves added with enzymatic bone paste, comprising the following steps:
[0056] (1) Raw material preparation
[0057] A. Prepare pigskin: clean the raw pigskin, remove the fat and hair on the skin, remove abnormal skins such as seals and belly milk skin, soak in 1.5% sodium lactate solution for 24 hours, take it out with 0.8% H 2 o 2 Solution bleaching, bleaching temperature 38 ° C, bleaching time 35 minutes, then cut the pigskin into pieces, boiled for 10 minutes, drained and cooled for later use;
[0058] B. Prepare raw meat: wash the fresh hoof meat, remove large pieces of fat, wash it with running water, drain it, mince the lean meat to get 0.3-0.5cm granular meat filling, take another fat meat, clean it, and cut it into 0.3-0.3- 0.5cm diced meat;
[0059] C. Prepare enzymatic bone paste:
[0060] Select fresh pig bones, remove the excess meat and other sundries on the bones, and clean them; put the cleaned pig...
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