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Small peptide and compositions formed by small peptide connection

A composition and short peptide technology, which is applied in the direction of non-active components of polymer compounds and peptides, can solve the problems of low efficiency of plasmid transfection, low efficiency of exosome recovery, and unclear conformation of targeted short peptide fusion expression

Active Publication Date: 2015-12-02
TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of this method, such as low plasmid transfection efficiency, unclear expression conformation of targeted short peptide fusion expression, and low exosome recovery efficiency, it seriously affects the development and application prospects of exosome as a transport carrier.

Method used

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  • Small peptide and compositions formed by small peptide connection
  • Small peptide and compositions formed by small peptide connection
  • Small peptide and compositions formed by small peptide connection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example A delivery composition formed by linking exosome and targeting peptide with a small peptide

[0065] The implementation process of this embodiment is as follows:

[0066] 1) In vitro recombination of the loop region of CD63 on the surface of the exosome, using the recombined loop region as a substrate for phage peptide library screening, to obtain small peptides that specifically bind to the loop region of CD63;

[0067] 2) Combine the obtained small peptide with the targeting peptide in a coupling manner, and connect the obtained chimeric short peptide to the loop region of CD63 on the surface of the exosome by co-incubating with the exosome;

[0068] 3) Load the antisense oligonucleotide drug MOE into the exosome by electroporation transformation method, and study the application of the exosome after the above targeted modification as a drug carrier.

[0069] Experimental part:

[0070] 1. Screening of CD63-specific binding small peptides

[0071] The exp...

Embodiment 2

[0194] Example Two small peptides link exosome and NIND to form a composition with an immune-promoting effect

[0195] The implementation process of this embodiment is as follows:

[0196] 1) In vitro recombination of the loop region of CD63 on the surface of the exosome, using the recombined loop region as a substrate for phage peptide library screening, to obtain a small peptide CP05 that specifically binds to the loop region of CD63;

[0197] 2) The resulting CP05 is coupled to the functional short peptide N1ND of the alarmin molecule (HMGN1), and the resulting chimeric short peptide is linked to the loop region of CD63 on the surface of the exosome by co-incubating with the exosome.

[0198] Experimental part:

[0199] 1. Screening of CD63-specific binding small peptides

[0200] This part is the same as the corresponding part of Embodiment 1, and will not be repeated here.

[0201] 2. In vitro verification of small peptides

[0202] This part is the same as the co...

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Abstract

The invention relates to the technical field of biological medicine projects, in particular to a small peptide and a screening method and application thereof. According to the application, the invention relates to a delivery composition composed of a small peptide connection carrier portion and a targeting peptide and a composition, with the function of promoting the immunity, composed of the small peptide connection carrier portion and a short peptide with the function of promoting the immunity.

Description

technical field [0001] The present invention relates to the technical field of biomedical engineering, in particular to a small peptide, and its screening method and application. In terms of application, it relates to a delivery composition composed of the small peptide connected to a carrier part and a targeting peptide, and the small peptide connected to a carrier. A composition with immune-promoting function composed of part and short peptide with immune-promoting function. Background technique [0002] exosome is a biological nanovesicle structure secreted by cells and body fluids, with a diameter of about 30-100nm, capable of carrying endogenous mRNA, miRNA and protein, etc. , the exchange of cellular substances and the presentation of antigens in cells and other biological processes. Recent studies have shown that exosome has the characteristics of carrying biological information of donor cells, which can be used as an indicator for clinical disease diagnosis. The exo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K47/42
Inventor 尹海芳高先军左冰峰冉宁董雪
Owner TIANJIN MEDICAL UNIV
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