A small peptide and composition formed by linking the small peptide
A technology of composition and linking carrier, which is applied in the direction of medical preparations, peptides, and pharmaceutical formulations of non-active ingredients, and can solve the problems of low plasmid transfection efficiency, affecting the development and application prospects of exosomes, and low recovery efficiency of exosomes
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Embodiment 1
[0064] Example 1 Delivery composition formed by connecting exosome and targeting peptide by small peptide
[0065] The implementation process of this embodiment is as follows:
[0066] 1) In vitro recombinant exosome surface CD63 loop region, using the recombinant loop region as a substrate to screen the phage peptide library to obtain small peptides that specifically bind to the CD63 loop region;
[0067] 2) Combine the obtained small peptide with the targeting peptide in a coupling manner, and the combined chimeric short peptide is connected to the loop region of CD63 on the surface of the exosome by co-incubation with the exosome;
[0068] 3) Load the antisense oligonucleotide drug MOE-RNA into the exosome using the electroporation transformation method, and study the application of the exosome modified by the above targeting as a drug carrier.
[0069] Experimental part:
[0070] 1. Screening of small peptides specifically binding to CD63
[0071] The experimental steps are as follow...
Embodiment 2
[0194] Example 2 A composition with immune-promoting effect formed by connecting exosome and NIND with small peptide
[0195] The implementation process of this embodiment is as follows:
[0196] 1) The loop region of CD63 on the surface of the exosome was reorganized in vitro, and the recombinant loop region was used as a substrate to screen the phage peptide library to obtain the small peptide CP05 that specifically binds to the loop region of CD63;
[0197] 2) Coupling the obtained CP05 with the functional short peptide N1ND of the alarm element molecule (HMGN1), and the combined chimeric short peptide is connected to the loop region of CD63 on the surface of the exosome by co-incubation with the exosome.
[0198] Experimental part:
[0199] 1. Screening of small peptides specifically binding to CD63
[0200] This part is the same as the corresponding part of Embodiment 1, and will not be repeated.
[0201] 2. In vitro verification of small peptides
[0202] This part is the same as t...
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