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Application of leatherleaf mahonia leaf extract to production of agricultural bactericide

A kind of narrow-leaf plant extract technology, applied in the field of pesticides, can solve the problem of less research on plants and achieve the effect of significant effect and good inhibitory effect.

Active Publication Date: 2015-12-02
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few studies on the antibacterial activity of the active substances of the plants of the genus Tensaurus in agriculture

Method used

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  • Application of leatherleaf mahonia leaf extract to production of agricultural bactericide
  • Application of leatherleaf mahonia leaf extract to production of agricultural bactericide
  • Application of leatherleaf mahonia leaf extract to production of agricultural bactericide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 : Extraction of Methanol Extract

[0029] The solvent impregnation method is used to extract the active substances of the ten major contributions of Anthophyllum angustifolia with methanol. The specific steps are as follows: dry the collected plant materials of the ten major contributions of Antenella angustifolia in the shade (55° C.), pulverize them with a pulverizer with a 40-mesh sieve, and soak them in methanol equivalent to 5 times the volume of the sample at room temperature. Stir 2-3 times, filter out the extract after 3 days, then add an equal amount of methanol, filter out the extract after 3 days, combine the two extracts, and use a rotary evaporator to concentrate under pressure at 55-60°C to It is viscous, scrape off the extract, and finally wash off the sticky extract with a small amount of methanol, and dry it at room temperature to obtain the methanol extract of the ten merits of Antena angustifolia. Weigh it and calculate the extraction rate,...

Embodiment 2

[0033] Example 2 : Inhibition of Phytopathogenic Fungi by Methanol Extracts of Attenuata

[0034] Under the condition of a concentration of 0.5g / L, the methanol extracts of the ten major contributions of the ankylosing leaves have certain antibacterial activities against 16 kinds of phytopathogenic fungi (see Table 1 for details), but the strengths are different. Among them, the antibacterial effect on corn leaf spot, corn leaf spot, cabbage black spot and banana curvularia leaf spot is the strongest, and the antibacterial rate is 100%; followed by citrus green mold and rice blast fungus, The bacteriostasis rate exceeds 85%; the bacteriostasis rate of Corynesporium nicotiana leaf spot, P. jasminum leaf spot, P. jasmine leaf spot, citrus resinous fungus and Jasmine branch blight are all 60% above; the antibacterial effect on rice sheath blight is the worst, and the antibacterial rate is 11.30%. Under the concentration of the solvent used to dissolve the extract, the growth o...

Embodiment 3

[0037] Example 3 : Inhibition of Phytopathogenic Bacteria by Methanol Extract of Angustifolia

[0038] Among the 8 kinds of phytopathogenic bacteria tested, the concentration of 30g / L methanol extract of the ten phytopathogenic bacteria had antibacterial activity against 6 kinds of phytopathogenic bacteria.

[0039] Table 2 Bacteriostatic activity of methanol extracts of the ten major meritorious service of Angus leaves on phytopathogenic bacteria

[0040]

[0041]

[0042] As shown in Table 2, it has the strongest antibacterial effect on rice bacterial stripe disease and rice bacterial blight, and the diameters of the inhibition zone are 43.50mm and 42.50mm respectively, and the difference between the two is not significant; Phytophthora solani, Ralstonia solanacearum and Prunus perforatum also have certain antibacterial effects, and the diameters of the inhibition zone are 16.50mm, 12.50mm, 7.67mm and 5.00mm respectively; Soft rot bacteria have no antibacterial effe...

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Abstract

The invention belongs to the technical field of pesticides, relates to an application of a leatherleaf mahonia leaf extract and particularly relates to an application of the leatherleaf mahonia leaf extract to the production of an agricultural bactericide. The application of the leatherleaf mahonia leaf extract is characterized in that the leatherleaf mahonia leaf extract is used for preparing the pesticides; and the leatherleaf mahonia leaf extract can be used for preventing and treating agricultural plant pathogenic fungi. The leatherleaf mahonia leaf extract has better inhibition effect on the plurality of types of agricultural plant pathogenic fungi and particularly has a remarkable effect of preventing and treating rice bacterial streak pathogenic bacteria; and the application provided by the invention lays a foundation for developing the leatherleaf mahonia leaf extract to a new botanical pesticide.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and relates to the use of the extract of the ten major contributions of Antenella angustifolia, in particular to the use of the extract of the ten major contributions of the narrow leaf in the production of agricultural fungicides. Background technique [0002] The Mahonia plant belongs to the Berberidaceae (Berberidaceae) and is a common medicinal and ornamental plant. There are about 100 species in the world, and about 50 species in my country, mainly distributed in West Asia, East Asia and Central America. It is mainly distributed in the south of the Yangtze River and Taiwan provinces in China, and it is distributed in all parts of Guangxi. There are mainly varieties of broad-leaved ten-song, narrow-leaf ten-song (also known as fine-leaf ten-song) and South China ten-song. The roots, stems, and leaves of the top ten merits all have high medicinal value. Among them, the roots and stems used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/08A01N43/90A01P1/00A01P3/00
Inventor 黎起秦袁高庆周荣金林纬
Owner GUANGXI UNIV
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