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A Strain of Ortho-Xylene Degrading Bacteria and Its Application

A technology of o-xylene and degrading bacteria, applied in the direction of bacteria, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problems of low degradation efficiency of o-xylene, achieve fast degradation speed, high tolerance concentration, good removal effect

Active Publication Date: 2018-07-24
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the problem of low degradation efficiency of ortho-xylene by ordinary microorganisms, and aims to provide an ortho-xylene-degrading bacterium that can efficiently degrade benzene series such as o-xylene, benzene and toluene, and realize the bioaugmentation of its coking wastewater deal with

Method used

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  • A Strain of Ortho-Xylene Degrading Bacteria and Its Application
  • A Strain of Ortho-Xylene Degrading Bacteria and Its Application
  • A Strain of Ortho-Xylene Degrading Bacteria and Its Application

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Pseudomonas ( Pseudomonas sp . ) Separation and identification of OX5 and its degradation performance of p-xylene

[0018] 1. Isolation and purification of strains

[0019] (1) Source of bacteria

[0020] The source of bacteria was taken from the long-term polluted soil in the sewage treatment plant of Wuhan Pingmei Wuhan Iron and Steel United Coking Co., Ltd., which was black in color. A total of 3 soil samples were collected at different locations.

[0021] (2) Isolation and purification of strains

[0022] Add the collected soil samples to distilled water and shake for 24 hours, after static centrifugation, take 5 ml supernatant and inoculate them in LB medium (peptone 1wt%, sodium chloride 1wt%, yeast extract 0.5wt%), and place at 30°C , 150 r / min constant temperature shaker enrichment culture for 24 h. Take 2 mL of the enriched bacterial solution, centrifuge, discard the supernatant, connect the bacterial cells to the inorganic salt medium conta...

Embodiment 2

[0033] Embodiment 2: environmental condition is to Pseudomonas ( Pseudomonas sp . ) Effect of OX5 degradability

[0034] 1. The influence of culture temperature

[0035] When the concentration of o-xylene is 1000mg / L, the pH is 7.0, the rotation speed of the shaker is 150 r / min, and the inoculum size is 2 vol%, the effect of temperature on the degradation of o-xylene is as follows: figure 2 Shown: In the range of 25-40 ℃, the strain OX5 has a good degradation effect on o-xylene, indicating that the bacteria has a wide adaptability to temperature. When the temperature was between 25 and 35°C, the degradation rate of the substrate increased as the temperature increased; but when the temperature reached 40°C, the degradation rate of the substrate by strain OX5 was lower than that at 35°C Rate. showed that Pseudomonas ( Pseudomonas sp . ) The optimum temperature of OX5 is 35℃.

[0036] 2. The influence of the initial pH value

[0037] When the concentration of o-xylene...

Embodiment 3

[0042] Embodiment 3: Pseudomonas ( Pseudomonas sp . ) OX5 Degradation of benzene series in coking wastewater

[0043] The bacterial suspension of strain OX5 was added to the diluted coking wastewater (COD=604mg / L) with an inoculation amount of 2vol%, and a certain concentration of benzene, toluene, o-xylene, m-xylene and p-xylene were added respectively , cultivated on a shaker at 35°C and 150r / min, measured the concentration of each benzene series remaining in the culture medium after 48 hours, and calculated the degradation rate of the corresponding benzene series as shown in Table 1: strain OX5 can decompose The three xylenes in the wastewater were completely degraded, and the degradation rates of p-benzene and toluene also reached more than 95%, indicating that the strain OX5 can better adapt to the environment in the coking wastewater, and can reduce the benzene series in the wastewater. Good degradation.

[0044] Table 1 Degradation effect of benzene series in coking...

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Abstract

The present invention disclosed a neighboring germinated bacteria and its application.The neighboring vitamin degradation OX5 provided by the present invention belongs to Pseudomonas SP. It is located in a typical cultivation center in China. The storage date is June 29, 2015, and the preservation number is CCTCC NO: M2015415.The bacteria is the Gram -negative bacteria. The colonies are round and slightly convex. The colonies are milky white, yellow in the middle, and the surface is moist and smooth.The bacteria can use neighbors as the only carbon source for growth and reproduction. When pure cultivation, the degradation rate of 48H to 1500mg / L neighboring t atr is as high as 98%, and the tolerance concentration of neighboring toluene reaches 2500mg / L.This bacteria can not only degrade the neighboring dysfamer and other benzene in the coking wastewater, but also reduce the COD in the coking biochemical water, which is used for biological reinforcement treatment of coking wastewater and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant bioaugmentation, and in particular relates to an o-xylene degrading bacterium and its application. Background technique [0002] Ortho-xylene is an important organic chemical raw material, mainly used in the production of phthalic anhydride, dyes, pesticides, paint solvents and drugs, etc., of which about 90% is used to produce phthalic anhydride, 2% is used to produce solvents, and only 1% Used to produce dye intermediates. O-xylene is highly toxic, and can irritate the skin and mucous membranes at low concentrations, and can penetrate into the body through the skin; at high concentrations, it can anesthetize the central nervous system and make people unconscious. Long-term exposure can affect liver and kidney function and cause neurasthenia syndrome. Using physical and chemical methods to treat o-xylene can achieve good results, but the cost is high and it is easy to cause seco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/34
Inventor 颜家保陈佩武文丽
Owner WUHAN UNIV OF SCI & TECH
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