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O-xylene degradation bacterium and application thereof

A technology of o-xylene and degrading bacteria, which is applied in the field of bioaugmentation of environmental pollutants, can solve the problems of low degradation efficiency of o-xylene, achieve fast degradation speed, high tolerance concentration, and good removal effect

Active Publication Date: 2015-11-25
WUHAN UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the problem of low degradation efficiency of ortho-xylene by ordinary microorganisms, and aims to provide an ortho-xylene-degrading bacterium that can efficiently degrade benzene series such as o-xylene, benzene and toluene, and realize the bioaugmentation of its coking wastewater deal with

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  • O-xylene degradation bacterium and application thereof
  • O-xylene degradation bacterium and application thereof
  • O-xylene degradation bacterium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Pseudomonas ( Pseudomonas sp . ) Separation and identification of OX5 and its degradation performance of p-xylene

[0018] 1. Isolation and purification of strains

[0019] (1) Source of bacteria

[0020] The source of the bacteria was taken from the long-term polluted soil in the sewage treatment plant of Wuhan Pingmei Wuhan Iron and Steel United Coking Co., Ltd., which was black in color. A total of 3 soil samples were collected at different locations.

[0021] (2) Isolation and purification of strains

[0022] Add the collected soil samples into distilled water and shake for 24 hours, after static centrifugation, take 5ml supernatant and inoculate them in LB medium (peptone 1wt%, sodium chloride 1wt%, yeast extract 0.5wt%), and place at 30°C, 150r / min constant temperature shaker enrichment culture for 24h. Take 2mL of the enriched bacterial solution, centrifuge, discard the supernatant, connect the bacterial cells to the inorganic salt medium cont...

Embodiment 2

[0033] Embodiment 2: Environmental conditions are to Pseudomonas ( Pseudomonas sp . ) Effect of OX5 degradability

[0034] 1. The influence of culture temperature

[0035] When the o-xylene concentration is 1000mg / L, the pH is 7.0, the shaking table speed is 150r / min and the inoculum size is 2vol%, the influence of temperature on the degradation of o-xylene is as follows figure 2 Shown: In the range of 25-40 ℃, the strain OX5 has a good degradation effect on o-xylene, indicating that the bacteria has a wide adaptability to temperature. When the temperature was between 25 and 35°C, the degradation rate of the substrate increased as the temperature increased; but when the temperature reached 40°C, the degradation rate of the substrate by strain OX5 was lower than that at 35°C Rate. showed that Pseudomonas ( Pseudomonas sp . ) The optimum temperature of OX5 is 35℃.

[0036] 2. The influence of the initial pH value

[0037] When the o-xylene concentration is 1000mg / L, t...

Embodiment 3

[0042] Embodiment 3: Pseudomonas ( Pseudomonas sp . ) OX5 Degradation of Benzene Series in Coking Wastewater

[0043] The bacterial suspension of strain OX5 was added to the diluted coking wastewater (COD=604mg / L) with an inoculation amount of 2vol%, and a certain concentration of benzene, toluene, o-xylene, m-xylene and p-xylene were added respectively , cultured on a shaker at 35°C and 150r / min, and after 48 hours, the concentration of each benzene series remaining in the culture medium was measured, and the degradation rate of the corresponding benzene series was calculated as shown in Table 1: the strain OX5 can decompose The three xylenes in the wastewater were completely degraded, and the degradation rates of p-benzene and toluene also reached more than 95%, indicating that the strain OX5 can better adapt to the environment in the coking wastewater, and can reduce the benzene series in the wastewater. Good degradation.

[0044] The degradation effect of benzene ser...

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Abstract

The invention discloses an o-xylene degradation bacterium and application thereof. The o-xylene degradation bacterium OX5 provided by the invention belongs to pseudomonas sp., which is preserved in China Center for Type Culture Collection on June 29, 2015 with preservation number of CCTCC NO: M2015415. The bacterium is a Gram-negative bacterium that colony is round, slightly convex, milk white in space around while yellow in middle and is smooth and wet in surface. The bacterium can grow and reproduce by taking O-xylene as a unique carbon source; during pure culture, the degradation rate on 1500mg / L of O-xylene reaches up to 98% at 48th hour, and tolerance concentration to the O-xylene is 2500mg / L. The bacterium not only can degrade O-xylene and other benzene series in coking wastewater but also can reduce COD (chemical oxygen demand) in discharged coking biochemical water; and the bacterium is applicable to the biological enhancement treatment of cooking wastewater and is extensive in application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant bioaugmentation, and in particular relates to an o-xylene degrading bacterium and its application. Background technique [0002] Ortho-xylene is an important organic chemical raw material, mainly used in the production of phthalic anhydride, dyes, pesticides, paint solvents and drugs, etc., of which about 90% is used to produce phthalic anhydride, 2% is used to produce solvents, and only 1% Used to produce dye intermediates. O-xylene is highly toxic, and can irritate the skin and mucous membranes at low concentrations, and can penetrate into the body through the skin; at high concentrations, it can anesthetize the central nervous system and make people unconscious. Long-term exposure can affect liver and kidney function and cause neurasthenia syndrome. Using physical and chemical methods to treat o-xylene can achieve good results, but the cost is high and it is easy to cause seco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/34
Inventor 颜家保陈佩武文丽
Owner WUHAN UNIV OF SCI & TECH
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