Extraction and purification process method for forsythiaside A in fructus forsythiae leaves

Abstract

The invention provides an extraction and purification process method for forsythiaside A in fructus forsythiae leaves. Fat-soluble pigment and water-soluble impurities in forsythiaside A extract are removed as much as possible before purification, the difficulty of following forsythiaside A purification is lowered, the chromaticity of the final product forsythiaside A is good, the purity reaches 95%-99%, and the extraction rate reaches 3.5%-5.5%. According to the technical scheme, the method includes the steps that 1, extraction is conducted; 2, extraction is conducted, wherein water is added to concentrate obtained in the step 1 for dissolution, an extracting solution is obtained, then the pH value is adjusted to be 6, petroleum ether is added, the fat-soluble pigment is removed through extraction, and then, most of the water-soluble impurities are removed through extraction through water-saturated normal butanol, wherein the use amount of water-saturated normal butanol is 0.5-2 times that of the extracting solution according to the volume ratio, the number of extraction times is 2-4, normal butanol is recovered, and forsythiaside A extract is obtained; 3, chromatographic column refining (fed to a 200-300-mesh silicagel column) is conducted, and coarse crystal substances are obtained; 4, recrystallization is conducted, and a finished product is obtained. The main innovation of the method is shown in extraction in the step 2.

Description

technical field [0001] The invention relates to a process for extracting and purifying forsythiaside A from forsythia leaves. Background technique [0002] At present, the extraction and purification methods of forsythiaside A have been reported. In the prior art, forsythiaside A is obtained by extracting with an organic solvent and then purifying it on a silica gel column or column chromatography. For example, the invention patent with publication number CN101215302A discloses a preparation method of forsythiaside A, which is Use organic solvent to extract, and then use column chromatography to purify (refine) to obtain forsythiaside A; although this method achieves the purpose of extracting and purifying (refining) forsythiaside A, but after using organic solvent to extract, directly extract The extract is subjected to column chromatography, and the extract also contains fat-soluble pigments and water-soluble impurities, which not only increases the difficulty and time of...

AI Technical Summary

Problems solved by technology

In the prior art, forsythiaside A is obtained by extracting with an organic solvent and then purifying it on a silica gel column or column chromatography. For example, the invention patent with publication number CN101215302A discloses a preparation method of forsythiaside A, which is Use organic solvent to extract, and then use column chromatography to purify (refine) to obtain forsythiaside A; although this method achieves the purpose of extracting and purifying (refining) forsythiaside A, but after using organic solvent to extract, directly extract The extract is subjected to column chromatography, and the extract also contains fat-soluble pigments and water-soluble impurities, which not only increases the difficulty and time of subsequent column chromatography, but also has many impurities in the finally obtained forsythiaside A, and forsythiaside The chroma of glycoside A is not good