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Mesenchymal stem cell excreted factor essence, and preparation method and application thereof

A technology for mesenchymal stem cells and secreting factors, applied in the field of mesenchymal stem cells secreting factor essence and its preparation, can solve problems such as allergies, and achieve the effects of preventing allergies, long-term biological activity, and avoiding damage

Inactive Publication Date: 2015-11-25
金凤华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defect that the essence in the prior art is prone to allergies, according to one aspect of the present invention, a mesenchymal stem cell (MSC) secreted factor essence that does not contain allergens is provided

Method used

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  • Mesenchymal stem cell excreted factor essence, and preparation method and application thereof
  • Mesenchymal stem cell excreted factor essence, and preparation method and application thereof
  • Mesenchymal stem cell excreted factor essence, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Example 1 Cord blood MSC isolation and culture

[0042]Mix the collected umbilical cord blood of the normal delivery baby within 8 hours and the 1640 medium produced by Gibco, and place the Ficoll separation medium produced by Tianjin Haoyang Biological Products Technology Co., Ltd. on the top of the separation medium according to the volume of 2:1. Centrifuge at 1800 rpm for 15 minutes to collect mononuclear cells, according to the number of monocytes, according to 1-5×10 6 / ml inoculated in MSC serum-free medium, at 37°C, 5% CO 2 , Cultivate under saturated humidity, induce adherence, change the medium after 72 hours, remove non-adherent cells, add fresh medium, and trypsin passage when the adherent cells are about 85-95% fused.

Embodiment 2

[0043] Example 2 Extraction of MSC Secreted Factors

[0044] Collect the culture nutrient supernatant with an MSC fusion degree of 85-95%, centrifuge at 2000 rpm for 10 minutes to remove residual cells in the medium, add the medium to an ultrafiltration tube of 2000da to 3000da, centrifuge at 5000rpm for 30min, and remove the medium After centrifugation, wash with sterile water once to completely remove the residual medium.

Embodiment 3

[0045] Preparation of Example 3 MSC Bioactive Factor Beauty Essence

[0046] After collecting the MSC factors and setting the concentration to 1.5mg / ml, human serum albumin was added as a beauty protection agent (so that the concentration of human serum albumin in the final essence was 10mg / mL), and the MSC beauty essence was obtained. The concentrations of various bioactive factors in the prepared essence are respectively:

[0047] Vascular endothelial growth factor, concentration range 11.4-0.45ng / ml

[0048] Epidermal growth factor, concentration range 6.2-1.0ng / ml

[0049] Fibroblast growth factor, concentration range 8.5-2.4ng / ml

[0050] Hepatocyte growth factor, concentration range 40.8-0.6ng / ml

[0051] Interleukin 6, concentration range 17.1-1.5ng / ml

[0052] Interleukin 8, concentration range 32.3-0.4ng / ml

[0053] MSC Beauty Essence can be directly applied to beauty parts in an amount of 1ml each time, or added to cosmetics at a ratio of 1:10, and homogenized t...

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Abstract

The invention provides mesenchymal stem cell excreted factor essence. The mesenchymal stem cell excreted factor essence comprises vascular endothelial growth factors with concentration range of 11.4-0.4ng / ml, epidermal growth factors with concentration range of 6.2-1.0ng / ml, fibroblast growth factors with concentration range of 8.5-2.4ng / ml, heaptocyte growth factors with concentration range of 40.8-0.6ng / ml, interleukin 6 with concentration range of 17.1-1.5ng / ml and interleukin 8 with concentration range of 32.3-0.4ng / ml. The essence prepared by the method has effects of nourishing skin, clearing away wrinkles and resisting aging, and has the advantages of free side effects and high stability; skins of users cannot be allergic easily; and the mesenchymal stem cell excreted factor essence can be used for whitening skin and resisting aging.

Description

technical field [0001] The method of the present invention relates to an essence and its preparation method and application, more specifically, relates to a mesenchymal stem cell (MSC) secretion factor essence and its preparation method and application. Background technique [0002] In the development history of beauty cosmetics, the first generation used natural animal and plant oils for pure physical protection of the skin; the second generation was cosmetics produced by oil-water mixing technology; the third generation was cosmetics containing pure natural animal and plant extracts, For example, animal and plant essences such as saponins, papaya, or deep-sea fish are added to cosmetics; the fourth generation is bionic cosmetics, which uses biotechnology to produce biological essences that are similar in structure to the human body and have affinity. With the development of the field of biotechnology, another major breakthrough has taken place in the fifth generation of pu...

Claims

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Application Information

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IPC IPC(8): A61K8/64A61Q19/08A61Q19/00
Inventor 金凤华
Owner 金凤华
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