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Preparation method of polypeptide feed additive

A feed additive and polypeptide feed technology, which is applied in the field of preparation of polypeptide feed additives, can solve problems such as unreasonable types and quantities of polypeptide amino acids, economic losses, etc., and help poultry and livestock resist infection by pathogenic bacteria, reduce morbidity, and improve immunity Effect

Active Publication Date: 2018-11-20
南宁荣港生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, animal nutritionists have found that the utilization of various amino acids in feed by animals is not completely affected by the level of a single limiting amino acid, so polypeptides made from a single protein often have unreasonable amino acid types and quantities. At the same time, there are some deficiencies in the prevention and treatment of poultry and livestock diseases by bioactive peptides. Once the disease prevention and treatment of poultry and livestock are not timely, it will cause major economic losses.

Method used

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  • Preparation method of polypeptide feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Step 1: cleaning the cicada slough and the honeycomb, the cleaning method of the cicada slough is: soaking the cicada slough in alcohol with a volume fraction of 95% for 10 minutes, and then rinsing twice with distilled water.

[0032] The cleaning method of the honeycomb is as follows: after soaking the honeycomb in a sodium bicarbonate solution with a mass fraction of 3% for 10 minutes, rinse it twice with distilled water.

[0033] Mix according to cicada slough and honeycomb mass ratio 1:1, pulverize, fry at 40 ℃ for 15 minutes and cool down, add a mass fraction of 1% sodium chloride solution to the resulting mixture, the quality of the sodium chloride solution is the same as that of the mixture The mass ratio of the mixed solution was 2:1. After the first mixed solution was obtained, the pH of the first mixed solution was adjusted to 4.5 with citric acid, refrigerated at 1° C. for 2 days, and then filtered at 1° C. to obtain the first filtrate.

[0034]Prepare a cul...

Embodiment 2

[0042] Step 1: cleaning the cicada slough and the honeycomb, the cleaning method of the cicada slough is: soaking the cicada slough in alcohol with a volume fraction of 95% for 20 minutes, and then rinsing it with distilled water for 3 times.

[0043] The cleaning method of the honeycomb is as follows: after soaking the honeycomb in a sodium bicarbonate solution with a mass fraction of 3% for 15 minutes, rinse it with distilled water for 3 times.

[0044] Mix cicada slough and honeycomb at a mass ratio of 1:1, pulverize, fry at 45°C for 20 minutes and cool, add a sodium chloride solution with a mass fraction of 3% in the resulting mixture, the quality of the sodium chloride solution is the same as that of the mixture The mass ratio of the mixed solution was 4:1. After the first mixed solution was obtained, the pH of the first mixed solution was adjusted to 5.0 with citric acid, refrigerated at 4° C. for 3 hours, and then filtered at 4° C. to obtain the first filtrate.

[0045]...

Embodiment 3

[0053] Step 1: cleaning the cicada slough and the honeycomb, the cleaning method of the cicada slough is: soaking the cicada slough in alcohol with a volume fraction of 95% for 15 minutes, and then rinsing twice with distilled water.

[0054] The cleaning method of the honeycomb is as follows: after soaking the honeycomb in a sodium bicarbonate solution with a mass fraction of 3% for 12 minutes, rinse it with distilled water for 3 times.

[0055] Mix cicada slough and honeycomb at a mass ratio of 1:1, pulverize, fry at 42°C for 18 minutes and cool, add a sodium chloride solution with a mass fraction of 2% in the resulting mixture, the quality of the sodium chloride solution is the same as that of the mixture The mass ratio of the mixture was 3:1. After the first mixed solution was obtained, the pH of the first mixed solution was adjusted to 4.8 with citric acid, refrigerated at 2° C. for 2.5 hours, and then filtered at 2° C. to obtain the first filtrate.

[0056] Prepare a cul...

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Abstract

The invention discloses a preparation method of a polypeptide feed additive. The preparation method comprises steps as follows: step one, cicada slough and a honeycomb are stir-fired and then ground, bifidobacteria are inoculated at the temperature of 1-4 DEG C after addition of a sodium chloride solution, the temperature is increased, and a first fermentation product is obtained through fermentation; step two, after distilled water is added to bean pulp and yeast is inoculated, the mixture is subjected to enzymolysis, and a mixture solution is obtained after enzyme deactivation; step three, polygonatum kingianum and radix stemonae are ground and decocted, and a second filtrate is obtained; step four, the first fermentation product, the mixed solution and the second filtrate are mixed and subjected to enzymolysis, then lactobacillus is inoculated, and the polypeptide feed additive is obtained. The prepared polypeptide feed additive contains multiple kinds of animal protein and plant protein, generates various polypeptides and amino acids after hydrolysis, contains a variety of amino acids, has high content of amino acids, and has functions of prevention and treatment of livestock influenza, resistance to infection of pathogenic bacteria and immunity enhancement, meanwhile, the laying rate of poultry and the lean meat percentage of livestock can be increased, and the economic benefits are increased.

Description

technical field [0001] The invention relates to a feed preparation method, more specifically, the invention relates to a preparation method of a polypeptide feed additive. Background technique [0002] Polypeptide is a compound formed by α-amino acids linked together by peptide bonds, and it is also an intermediate product of protein hydrolysis. Peptides with biological activity, that is, active peptides, have the characteristics of easy digestion and absorption, anti-fatigue, lowering blood pressure, lowering cholesterol, promoting mineral absorption, promoting fat metabolism, promoting microbial fermentation and low allergenicity. [0003] Studies in recent decades have shown that bioactive peptides have a wide range of nutritional and physiological regulation effects on animals, and can be completely degraded in animals. They are safe to use, have no toxic side effects, and have a wide range of applications. The requirements of development and healthy breeding of livesto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/26A23K10/12A23K10/37A23K10/14A23K10/30
CPCY02P60/87
Inventor 黄赵才
Owner 南宁荣港生物科技有限公司
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