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Method and kit for detecting group B streptococcus infection

A technology for detecting reagents and streptococcus, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, etc. It can solve the problems of no literature and products for detecting group B streptococcus, and no discovery, and achieve visual identification results Intuitive and accurate, good performance, simple structure effect

Inactive Publication Date: 2015-11-18
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the dry chemical enzyme rapid detection technology produced by the high sensitivity, high specificity and fast reaction of the enzyme reaction combined with the local enrichment technology of the solid phase carrier has been widely used in recent years due to its advantages of simple operation and short detection time. application, but up to now, no documents and products using this technology to detect group B streptococcus have been seen, nor have they been found to use chromogenic substrates instead of sodium hippurate in the sodium hippurate test, especially the rapid sodium hippurate test. literature reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Preparation of detection reagent paper

[0018] Prepare 100mM citric acid-sodium citrate as buffer and sample diluent (pH4.0, containing 1% [v / v] TritonX-100); 3,3-bis(4-hydroxy-5-isopropyl -2-Tolyl)-3-Hydroxy-benzoylglycinate was dissolved with the above prepared 100mM citric acid-sodium citrate to make 3,3-bis(4-hydroxy-5-isopropyl-2-tolyl) )-3-Hydroxy-benzoylglycinate sodium concentration reaches 2mg / ml, mix evenly, add on the paper sheet of 5mm side length by 15ul / sheet, can obtain colorless detection reagent paper sheet after drying overnight.

[0019] 2. Detection method

[0020] 1) Put the secretion sample test tube into a clean test tube, add 300~400ml of the 100mM citric acid-sodium citrate solution prepared above, and stir the test test well to fully dissolve the secretion in the test test tube into the sample diluent ;

[0021] 2) Add 40 μl of the above-mentioned dissolved secretion sample to the colorless detection reagent paper, shake and mix evenly, ...

Embodiment 2

[0027] 1. Preparation of detection reagent paper

[0028] Prepare 100mM acetic acid-sodium acetate as a buffer and sample diluent (pH4.0, containing 0.8% [v / v] TritonX-100); add 2-[[4-(dimethylamino)phenyl]azo ]-sodium benzoylglycinate is dissolved with the 100mM acetic acid-sodium acetate prepared above, so that the concentration of 2-[[4-(dimethylamino)phenyl]azo]-sodium benzoylglycinate reaches 1.5mg / ml, Mix evenly, add 15ul / sheet to a paper sheet with a side length of 5mm, and dry overnight to obtain a colorless or very light red detection reagent paper sheet.

[0029] 2. Detection method

[0030] 1) Put the secretion sample test tube into a clean test tube, add 300~400ml of the 100mM citric acid-sodium citrate solution prepared above, and stir the test test well to fully dissolve the secretion in the test test tube into the sample diluent ;

[0031] 2) Add 40 μl of the above-mentioned dissolved secretion sample to the colorless detection reagent paper, shake and mix ev...

Embodiment 3

[0035] 1. Preparation of detection reagent paper

[0036] Prepare 50mM acetic acid-sodium acetate as buffer and sample diluent (pH5.2, containing 0.8% [v / v] TritonX-100); 2-[[4-(dimethylamino)phenyl]azo ]-Benzoylglycine was dissolved with dimethyl sulfoxide at a final concentration of 5%, and then added to the 50mM acetic acid-sodium acetate solution prepared above to dilute and mix to make 2-[[4-(dimethylamino)phenyl] The concentration of azo]-benzoylglycine reaches 1.0 mg / ml, mix well, add 15ul / sheet to a paper sheet with a side length of 5mm, and dry overnight to obtain a colorless or very light red detection reagent paper sheet.

[0037] 2. Detection method

[0038] 1) Put the secretion sample test tube into a clean test tube, add 300~400ml of the 100mM citric acid-sodium citrate solution prepared above, and stir the test test well to fully dissolve the secretion in the test test tube into the sample diluent ;

[0039] 2) Add 40 μl of the above-mentioned dissolved secreti...

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PUM

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Abstract

The invention discloses a method for detecting group B streptococcus infection, which is characterized in that a detection reagent prepared from hippuric acid or hippurate analogue as a chromogenic substrate and a buffer solution is adopted, and hippuricase in group B streptococcus can be used for specifically recognizing glycine and benzoic acid in the chromogenic substrate and hydrolyzing amido bonds for linking glycine and benzoic acid to change the color of the chromogenic substrate, so that whether group B streptococcus is infected or not in a detected sample is judged. The invention also discloses a kit for detecting group B streptococcus infection. The detection method disclosed by the invention is simple, professional training required for the bacterial streaking technology is not needed, identification results can be obtained only by simple sample adding and short-time incubation, the detection time is far shorter than the time required for culture by a culture medium, and visual identification results are intuitive and accurate. The kit disclosed by the invention is simple in structure, convenient in operation and good in performance. Statistics show that by adopting the kit for detection, the positive predictive value is more than 85%, and the negative predictive value is more than 97%.

Description

Technical field [0001] The present invention involves dry chemicals fast inspection technology, especially the method of testing the use of cadre chemical fast inspection technology to detect the B -group binococcus infection. The invention also involves detecting the B -group Bacteria infection kit. Background technique [0002] Group B Chain Bacteria (GBS), also known as B -tribe or S.AGALACTIAE (S.Agalactiae), is also anaerobic., Various lengths; 36 ± 1 ℃ on the blood agar tablet for 18 ~ 24h, forming gray -white, smooth surface, creamy, circular, and β hemolysis, there are also a few strains without β hemolytic ring; tental test negative, negative, and tested, negative, and negative, tentative, negative, negative, and negative, and negative, and the trials of the trials of the trialSodium uric acid test is positive and CAMP test positive. [0003] Bun Bangeng bacteria live in human vagina and rectums normally. It is a conditional pathogenic bacteria. About 1 / 3 of the adult in...

Claims

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Application Information

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IPC IPC(8): C12Q1/34C12Q1/14
Inventor 王则宇马建军赵磊王利英杨洋付光宇吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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