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Method for preparing collagen

A collagen and pro-collagen technology, which is applied in the field of biological food, can solve the problems of many collagen impurities, low purification rate, affecting the quality of collagen, etc., and achieve the effects of increasing the purification rate and purity, simple process and easy operation.

Inactive Publication Date: 2015-11-18
FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
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  • Abstract
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Problems solved by technology

Because collagen has the effects of firming and wrinkling, moisturizing, repairing tissue, whitening and blemishes, etc., it has been used by people for food and medicine. Collagen is generally extracted from pigskin and cowhide. Due to the influence of process conditions, the purification rate is low, and there are many impurities in the extracted collagen, which seriously affects the quality of collagen

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  • Method for preparing collagen

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Embodiment Construction

[0041] Collagen preparation steps of the present invention are:

[0042] a. Homogenate digestion:

[0043] Bovine dermal tissue thawed

[0044] Take the frozen bovine dermis tissue, add ultra-pure water to soak until the bovine dermis thaws into loose tissue particles, stir evenly with a glass rod, and form a paste; add 10 times the volume of ultra-pure water to the pasty tissue particles, Homogenize 3 times according to the operating procedures of the tissue masher until completely suspended; mince the tissue particles evenly, add ultrapure water until the content of the tissue is 25 g / L, and mix well;

[0045] Add glacial acetic acid to the tissue suspension to adjust the solution concentration to 0.5 mg / ml;

[0046] Digestion: Weigh pepsin, dissolve pepsin in 0.5M acetic acid to make 10mg / ml pepsin solution, then transfer to tissue suspension to 0.1mg / ml, that is, add 1ml pepsin to every 100ml homogenate Solution, mix well, digest at room temperature for 7-10 days;

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Abstract

The invention belongs to the technical field of biological food, and aims to provide a method for preparing collagen with the skin of cattle as the main raw material. The method for preparing the collagen includes the steps of homogenization digestion, adsorption filtration, ultrafiltration concentration and buffer solution change, ion-exchange column chromatography, ultrafiltration concentration and preservation solution change, and filtration sterilization for preparation of a collagen stock solution, wherein the collagen is obtained after protein precipitation, centrifugation and fiber homogenization. The process is simple and easy to implement; by using serous fluid obtained after treatment of the skin of cattle for extracting the collagen through the process, the purification rate and purity of the collagen can be obviously increased.

Description

Technical field [0001] The present invention belongs to the field of biological food technology. Background technique [0002] Collagen is the most abundant protein in the human body, accounting for more than 30%of the total protein of the whole body.Collagen contains amino acids such as glycine, proline, hydroxyticine for the human body.Collagen is the most important part of the extracellular matrix.Because collagen has the functions of firming, moisturizing, moisturizing, repairing tissue, whitening and light spots, it has been eaten and medical. Collagen is generally extracted in pork skin, cowhide, etc.The influence of process conditions is low, and the purification rate is low, and the extracted collagen has more impurities, which seriously affects the quality of collagen. Invention content [0003] The purpose of the present invention is to provide a preparation method to prepare collagen with cowhide as the main raw material. [0004] The preparation steps of the collagen...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/18
Inventor 杨中万彭天骥张鹰黄海军田会全刘建军孙淑娟陈云声
Owner FILLDERM (CHANGCHUN) MEDICINE BIOLOGY TECH CO LTD
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