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Application of radix bupleuri total saponin and monomer saponin thereof in pharmacy

A technology of saikosaponin and total saponin, applied in the field of medicine, can solve problems such as drug application without obesity, and achieve the effect of inhibiting occurrence and development

Inactive Publication Date: 2015-11-18
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But for the total saikosaponins or saikosaponin monomer compounds to 5-HT 2C There are no literature reports on receptor agonism and appetite suppression, and there is no report on their application in the preparation of drugs for the prevention or treatment of obesity

Method used

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  • Application of radix bupleuri total saponin and monomer saponin thereof in pharmacy
  • Application of radix bupleuri total saponin and monomer saponin thereof in pharmacy
  • Application of radix bupleuri total saponin and monomer saponin thereof in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Preparation of total saikosaponins and monomer saikosaponins:

[0060] Take Bupleurum chinense DC medicinal material (6.0kg) and pulverize, reflux and extract with 70% EtOH for three times, combine the extracts, concentrate to a small volume, perform D101 macroporous resin column chromatography, elute with water, 70% EtOH, EtOH successively; wash with 70% EtOH Defractionated Rp-18 reverse phase column chromatography, MeOH-H 2 O (1:9→10:0) was eluted to obtain the total saikosaponins (150g); the total saikosaponins were separated by various column chromatography to obtain the following monomer saikosaponins 3-O-β-D-fucopyranosylsaikogeninf (1,60mg), saikosaponina (saikosaponina,2,2.1g), 6”-O-acetylated saikosaponina (6”-O-acetylsaikosaponina,3,84mg), 3”,6”-di -O-saikosaponin a (3”, 6”-di-O-acetylsaikosaponina, 4,0.2g), saikosaponin d (saikosaponind, 5,234mg), saikosaponin e (saikosaponine, 6,102mg), Bupleurum saponin b 1 (saikosaponinb 1 ,7,330nmg), saikosaponin g (s...

Embodiment 2

[0063] Dissolve the prepared total saikosaponins or monomer saikosaponins or saikosaponina (2) with a small amount of DMSO, add water for injection as usual, fine filter, potting and sterilize to make an injection.

Embodiment 3

[0065] Dissolve the prepared total saikosaponins or monomer saikosaponins or saikosaponina (2) with a small amount of DMSO, dissolve it in sterile water for injection, stir to dissolve, and filter with a sterile suction filter funnel , and then aseptically finely filtered, divided into ampoules, freeze-dried at low temperature and sealed aseptically to obtain a powder injection.

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PUM

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Abstract

The invention provides an application of radix bupleuri total saponin and monomer saponin thereof in preparation of medicines for preventing and treating obesity, and a pharmaceutical composition taking the radix bupleuri total saponin and the monomer saponin as active components. A pharmacological experiment proves that the radix bupleuri total saponin and a monomer compound thereof have obvious agonist activity on a 5-HT2C receptor, has obvious effects of inhibiting appetite and inhibiting weight gain on SD rats in an animal experiment, and can be applied to prevention and treatment of obesity.

Description

Technical field: [0001] The invention belongs to the technical field of medicines. Specifically, the present invention relates to extracting and separating total saponins and monomeric saponins from Bupleurum bupleuri, a traditional Chinese medicine, and their application in preparing medicines for preventing and treating obesity. Background technique: [0002] Obesity refers to excessive adipose tissue in the body. Generally, if the body fat content exceeds 30% in adult women and 20% to 25% in adult men, it can be confirmed as obesity. Obesity is closely related to the occurrence of various diseases such as hypertension, hyperlipidemia, and diabetes. Its impact on health has led many countries to list it as a disease of preventable death. The weight-loss drugs currently on the market mainly work by suppressing appetite, increasing energy consumption and inhibiting intestinal digestion and absorption, such as sibutramine and orlistat. However, the toxic side effects of the...

Claims

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Application Information

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IPC IPC(8): A61K36/233A61K31/704A61P3/04
Inventor 陈纪军孙长利耿长安黄晓燕马云保张雪梅
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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