Method for increasing phaffia rhodozyma astaxanthin

Abstract

The invention discloses a method for increasing phaffia rhodozyma astaxanthin, which comprises the following steps: adjusting pH value of a phaffia rhodozyma broth to 9.0-11.5, controlling the temperature at 35-60 DEG C, stirring for 80-150r / min, breaking wall for 1.0-5.0; neutralizing the pH value to 6.0-7.5, mixing with water having 0.5-1 times of volume, centrifuging and concentrating to obtain the concentrated wall breaking phaffia rhodozyma astaxanthin slurry; then uniformly mixing with a proper amount of complex formulation anti-oxidant and performing spray drying, wherein the air intake temperature is 170-185 DEG C, air-out temperature is 70-85 DEG C, and obtaining the astaxanthin dry powder, wherein the moisture of the dry powder is less than 8%, and spray loss amount is less than 4.0%. According to the invention, the method is simple and practical, total pigment can be increased by 10-12%, wall breaking time is short, cost is low, and the method is suitable for industrial production, shelf time of the produced astaxanthin dry powder is longer, taste is fragrant and unique, the phaffia rhodozyma astaxanthin has good palatability, and the dry powder can be widely used in animal feed industry.

Description

technical field [0001] The invention relates to a method for improving Phaffia rhodozyme astaxanthin. Background technique [0002] Phaffia red yeast (Phaffiarhodozyma) is the only species of the kingdom Fungi, Phylum Fungi, Myxomycota, Cryptococcus family, and the genus Phaffia. Obligate aerobic bacteria, the mode of reproduction is asexual budding. The fermentation of the bacteria can produce more than 10 kinds of carotenoids, mainly astaxanthin, β-carotene, γ-carotene, etc., of which astaxanthin accounts for 60% to 85%. Cells need a lot of oxygen to produce carotenoids. It can use a variety of carbon sources for fermentation. Glucose and sucrose are the best carbon sources. The most suitable growth temperature is 20-28°C and the optimum pH is 5.0-6.9. Phaffia rhodozyme produces acid during aerobic fermentation and releases carbon dioxide and simultaneously synthesize astaxanthin. [0003] Phaffia rhodozyme is mainly single-celled and can sometimes form pseudohyphae. ...

AI Technical Summary

Problems solved by technology

[0006] At present, astaxanthin technical preparation and production method is the first to promote microbial fermentation, but Phaffia astaxanthin is generally much lower than Haematococcus pluvialis astaxanthin content, especially astaxanthin is sensitive to light, heat, oxygen, etc., so in addition to In addition to improving its fermentation level, the method of indirectly increasing the astaxanthin content of Phaffia yeast by chemically thinning the thickness of the yeast cell wall and its application has not been reported at home and abroad.

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