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Method for detecting dopamine on basis of nanoparticle label oxidation-reduction cycle

A nanoparticle, cyclic detection technology, applied in the direction of electrochemical variables of materials, chemical analysis using catalysis, etc., can solve the problems of inability to meet fast and simple detection requirements and high operating costs.

Active Publication Date: 2015-11-11
凯惠睿智生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these methods require large-scale instruments and have disadvantages such as high operating costs, they cannot meet the requirements of rapid and simple detection. It is necessary to establish a new method for simple and rapid detection of dopamine.

Method used

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  • Method for detecting dopamine on basis of nanoparticle label oxidation-reduction cycle
  • Method for detecting dopamine on basis of nanoparticle label oxidation-reduction cycle
  • Method for detecting dopamine on basis of nanoparticle label oxidation-reduction cycle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 The effect of the interaction time of probe and dopamine on electrochemical signal

[0040] Experiments have found that the action time of the probe and dopamine aptamer should be appropriate, and the action time is not as long or as short as possible. Such as figure 2 As shown, af represents the electrochemical signal curve of 0.5 hour, 1 hour, 1.5 hour, 2.0 hour, 2.5 hour and 3.0 hour respectively. It can be seen from the figure that the electrochemical signal reaches a larger value when the action time is 2.5 hours After increasing the action time, the electrochemical signal does not change significantly, so the action time of the probe and dopamine aptamer is selected to be 2.5h.

Embodiment 2

[0041] Example 2 The influence of the incubation time of the electrode on the electrochemical response signal

[0042] The incubation time of the gold nanoparticle and graphene oxide modified gold electrode and the gold-platinum composite nanoparticle probe after the action of dopamine has an important influence on the size of the electrochemical signal. Such as image 3 As shown, a-h respectively represent the electrochemical signal curve when the incubation time is 0.5h, 1.0h, 1.5h, 2.0h, 2.5h, 3.0h, 3.5h and 4.0h. It can be seen from the figure that as the time increases from 0.5h to 3.0h, the response signal will gradually increase. But when the incubation time continued to increase from 3h, the response signal decreased instead, so an incubation time of 3.0h was adopted.

Embodiment 3

[0043] The sensitivity comparison of the method in Example 3

[0044] The graphene oxide modified gold electrode is used to replace the gold nanoparticle and graphene oxide modified gold electrode, and dopamine is detected according to the above-mentioned (4) analysis and determination procedure. The linear range is 7.0×10 -8 mol / L~1.0×10 -6 mol / L, detection limit is 3.0×10 -8 mol / L. Taking the detection limit as a comparison, the sensitivity of gold nanoparticles and graphene oxide-modified gold electrodes is 60 times that of graphene oxide-modified gold electrodes, indicating that the presence of gold nanoparticles greatly improves the sensitivity of detection, and is effective in detecting dopamine. Obvious enhancement effect.

[0045]

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Abstract

The invention belongs to the field of electrochemical sensors and relates to a method for detecting dopamine on the basis of a nanoparticle label oxidation-reduction cycle. According to the method, gold platinum composite nanoparticles are connected to dopamine aptamer complementary sequence single-stranded DNA to form a probe, the dopamine aptamer is added into the probe, so that double-stranded DNA modified gold platinum composite nanoparticles are obtained, when a dopamine-containing sample solution is added into the double-stranded DNA modified gold platinum composite nanoparticles, dopamine is specifically bonded with the dopamine aptamer and the probe is released; the released probe is captured by gold nanoparticles and a graphene oxide modified gold electrode, and the catalysis effect of gold platinum composite nanoparticles is used to generate an electrochemical signal to realize detection of dopamine. The method provided by the invention has the advantages of being simple and high in sensitivity.

Description

Technical field [0001] The invention belongs to the field of electrochemical sensors, and particularly relates to a method for detecting dopamine based on a nanoparticle marking redox cycle. Background technique [0002] Dopamine is secreted by the hypothalamus and is mainly responsible for the transmission of emotional information in the brain. In medicine, dopamine is often used to treat depression. If the content of dopamine in the human body is insufficient, the body will lose the instinct to control muscles. A severe lack can cause Alzheimer's, schizophrenia, Parkinson's syndrome, etc. The detection of dopamine mostly uses liquid chromatograph analysis, fluorescent label analysis, high performance liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry and other analytical methods. Because these methods require large-scale equipment, they have the disadvantages of expensive operation, and cannot meet the requirements of quick and simple detection. It i...

Claims

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Application Information

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IPC IPC(8): G01N31/10G01N27/26G01N27/30
Inventor 混旭张跃谢国亮
Owner 凯惠睿智生物科技(上海)有限公司
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