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Isolation and purification method, culture medium and preparation method of endophytic bacteria in peony vegetative organs

A technology for vegetative organs and endophytic bacteria, applied in the direction of bacteria, etc., to achieve good promotion and application value, simple preparation method, and rich strain types

Inactive Publication Date: 2020-04-07
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the research on the endophytic bacteria of peony, and the research on the endophytic bacteria of vegetative organs is even blank. Therefore, the medium formula for the isolation of endophytic bacteria in peony is innovated, and the formula of the medium for the isolation of endophytic bacteria in peony and its preparation technology are clarified. , which can not only expand the screening approach of microbial resources, provide technical support for downstream research, but also increase the diversity of strains screened from endophytic bacteria in peony, enrich the theory of development and utilization of endophytic bacteria in peony, minimize unnecessary repeated screening, and provide rare species Screening out provides more possibilities, which is of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] In this example, the medium for the isolation and purification of endophytic bacteria in peony vegetative organs, the composition of each 1000mL medium is: 2g of golden cicada dry powder, 5g of yeast powder, 2g of gellan gum, 0.5g of trehalose, 0.3g of inulin, NaCl 9g, peony rhizosphere soil extract 200mL, agar powder 16g, the balance is distilled water, pH7.0.

[0042] The preparation method of the culture medium for separating and purifying endophytic bacteria from peony vegetative organs comprises the following steps:

[0043] 1) Preparation of Jinchan dry powder:

[0044] Weigh an appropriate amount of mature nymphs of the golden cicada, sterilize them with high pressure steam at 121°C for 20 minutes, dry them in a constant temperature drying oven at 90°C overnight, cut off the forefeet, grind them with a mortar until there are no obvious particles, and pass 160°C Mesh sieve to get golden cicada dry powder;

[0045] 2) Preparation of peony rhizosphere soil extract...

Embodiment 2

[0058] In this embodiment, the medium for the separation and purification of endophytic bacteria in peony vegetative organs, the composition of each 1000mL medium is: 1.5g of golden cicada dry powder, 6g of yeast powder, 2.5g of gellan gum, 1g of trehalose, 0.1g of inulin, NaCl 8g, peony rhizosphere soil extract 250mL, agar powder 18g, the rest is distilled water, pH7.0.

[0059] The preparation method of the culture medium for separating and purifying endophytic bacteria from peony vegetative organs comprises the following steps:

[0060] 1) Preparation of Jinchan dry powder:

[0061] Weigh an appropriate amount of mature nymphs of the golden cicada, sterilize them with high pressure steam at 121°C for 20 minutes, dry them in a constant temperature drying oven at 90°C overnight, cut off the forefeet, grind them with a mortar until there are no obvious particles, and pass 160°C Mesh sieve to get golden cicada dry powder;

[0062] 2) Preparation of peony rhizosphere soil extr...

Embodiment 3

[0071] The separation and purification medium of endophytic bacteria in peony vegetative organs in this example, the composition of each 1000mL medium is: 2.5g of golden cicada dry powder, 4g of yeast powder, 1.5g of gellan gum, 0.2g of trehalose, 1g of inulin, NaCl 10g, peony rhizosphere soil extract 150mL, agar powder 14g, the rest is distilled water, pH7.0.

[0072] The preparation method of the culture medium for separating and purifying endophytic bacteria from peony vegetative organs comprises the following steps:

[0073] 1) Preparation of Jinchan dry powder:

[0074] Weigh an appropriate amount of mature nymphs of the golden cicada, sterilize them with high-pressure steam at 121°C for 20 minutes, and dry them overnight in a constant temperature drying oven at 90°C. Mesh sieve to get golden cicada dry powder;

[0075] 2) Preparation of peony rhizosphere soil extract:

[0076] Take some peony rhizosphere soil, remove impurities by hand, and pass through a 60-mesh siev...

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Abstract

The invention discloses a separation and purification method, a culture medium and a preparation method of peony vegetative organ endophytic bacteria and belongs to the technical field of plant endophytic bacteria separation and cultivation. Each 1000mL of the culture medium is composed of 1.5-2.5g of dry golden cicada powder, 4-6g of yeast powder, 1.5-2.5g of jilan gelarose, 0.2-1g of trehalose, 0.1-1g of synanthrin, 8-10g of NaCl, 150-250mL of peony rhizosphere leaching liquid, 14-18g of agar powder and the balance distilled water. The culture medium is precise and appropriate in material matching integrally and suitable for separation and cultivation of vegetative organ endophytic bacteria of roots, stems and leaves of peony and can be used for scribing purification; compared with conventional culture media, the culture medium has the advantages that bacterial strains which are richer in type can be separated and cultivated, and diversity advantage is obvious. The preparation method is simple and convenient in operation and has good popularization and application value.

Description

technical field [0001] The invention relates to plant endophytic bacteria separation and cultivation technology, in particular to a method for separation and purification of endophytic bacteria in peony vegetative organs, a culture medium used in the separation and purification operation and a preparation method thereof. Background technique [0002] Plant endophytes generally refer to a type of microorganisms that live in plant tissues at a certain stage of their life history and do not cause obvious disease symptoms to plant growth and development, such as fungi, bacteria, and actinomycetes. Plant endophytes are often a stable symbiotic or parasitic relationship formed by long-term co-evolution of different types of microorganisms in a specific habitat. Some physiological activity characteristics of endophytes are often different from indigenous microbial groups. Screening specific strains, A good source of material for functional enzyme-producing strains or agricultural b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 孙会忠宋月芹王小东薛娴侯小改
Owner HENAN UNIV OF SCI & TECH
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