Establishment of Immunosuppressive Model in Rhesus Monkeys

Abstract

The invention relates to establishment of a rhesus monkey immunosuppression model, and provides an application of cyclophosphamide in preparing the rhesus monkey immunosuppression model. Specifically, cyclophosphamide with different dosages are selected to carry out intravenous injection on the rhesus monkey, then the suitable immunosuppression molding dosage is determined, and a disease model consistent with the variation tend of various immune indices under human immunosuppression state is established, wherein the optimal use method and the optimal use amount of cyclophosphamide are as follows: intravenous injection is carried out at 15mg / kg each day. The invention further provides a method for preparing the rhesus monkey immunosuppression model, and an immunosuppression animal module prepared by the method. The animal mold can be used for the fields of biotechnology new drug research and development, immunosuppression mechanism study, virus infection modeling and the like, which can not be completed by rodent model, and provides a more ideal and more reliable experimental animal models and a perfect evaluation system for the above application compared with the rodent model.

Description

technical field [0001] The invention specifically relates to the application of cyclophosphamide in preparing a rhesus monkey immunosuppression model, the rhesus monkey immunosuppression model and a preparation method thereof. Background technique [0002] The establishment and application of animal disease models are becoming more and more extensive. At present, most of them focus on rodents, but their physiological functions are far from those of humans. Using non-human primate rhesus monkeys to establish experimental animal disease models has unparalleled advantages. Rhesus monkeys are highly similar to humans in terms of biochemical metabolism, reproductive physiological characteristics, and tissue structure, and have 98% genetic material with humans. % or so homology. And it has been used as a biomedical research model for nearly a hundred years. For example, the establishment of Parkinson's disease, tuberculosis, liver transplantation and other disease models, the dat...

AI Technical Summary

Problems solved by technology

But at present, cyclophosphamide is more used as an immunosuppressant, and a large number of studies have shown that it can inhibit the cellular and humoral immune responses of animals, resulting in immunosuppression of animals.

[0004] Due to the great differences in physiological functions between rodents and primates, the mouse immunosuppressive model established by Gao Peng et al. is used as a reference for the research and development of new biotechnology drugs for human use, the study of human immunosuppressive mechanism and the establishment of virus infection models. Evaluation system, its reliability is questionable

In addition, according to the search, there is currently no report on the use of non-human primates to establish an immunosuppressive model

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