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Preparation method for hemoglobin molecularly imprinted polymers through autocatalysis of ATRP

A technology for imprinting polymer and hemoglobin, which is applied in the field of preparing hemoglobin imprinting polymers, can solve the problems of toxicity of metal complexes, increase the preparation cost, difficult preservation of low-valence metal complexes, etc., and achieves good detection and good identification. Effect

Active Publication Date: 2015-11-04
内蒙古澳菲利食品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The required low-valence metal complexes are sensitive to air and are not easy to store;
[0007] 2. The required low-valence metal complexes have certain toxicity to biological macromolecules such as proteins;
[0008] 3. The post-treatment process for catalyst removal is relatively complicated, which increases the preparation cost

Method used

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  • Preparation method for hemoglobin molecularly imprinted polymers through autocatalysis of ATRP
  • Preparation method for hemoglobin molecularly imprinted polymers through autocatalysis of ATRP
  • Preparation method for hemoglobin molecularly imprinted polymers through autocatalysis of ATRP

Examples

Experimental program
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Effect test

Embodiment 1

[0022] First according to prior art, utilize initiator-bromine-containing compound to modify electrode surface, then proceed according to the following steps:

[0023] a. The bromine-containing compound modified gold electrode is used as the working electrode, the saturated calomel electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode, and the working electrode, the reference electrode and the counter electrode constitute a three-electrode system;

[0024] b. Put the three-electrode system into a solution prepared by 0.28g acrylamide, 0.093g N,N-methyleneisoacrylamide and 0.019g hemoglobin molecule, and apply a constant voltage between the working electrode and the reference electrode. Potential -0.6V, applied for 240min;

[0025] c. Put the working electrode into the eluent composed of 10% (v / v) acetic acid and 10% (v / v) sodium lauryl sulfate solution, soak for 120min, and elute hemoglobin molecules to obtain hemoglobin impr...

Embodiment 2

[0038] First according to prior art, utilize initiator-bromine-containing compound to modify electrode surface, then proceed according to the following steps:

[0039] a. The graphite electrode modified with bromine compound is used as the working electrode, the mercurous sulfate electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode, and the working electrode, the reference electrode and the counter electrode constitute a three-electrode system;

[0040] b. Put the three-electrode system into a solution prepared by 0.71g acrylamide, 0.19g N,N-methyleneisoacrylamide and 0.075g hemoglobin molecule, and apply a constant voltage between the working electrode and the reference electrode. Potential -0.3V, applied for 60min;

[0041] c. Put the working electrode into the eluent composed of 10% (v / v) acetic acid and 10% (v / v) sodium lauryl sulfate solution, soak for 120min, and elute hemoglobin molecules to obtain hemoglobin imprinte...

Embodiment 3

[0043] First according to prior art, utilize initiator-bromine-containing compound to modify electrode surface, then proceed according to the following steps:

[0044] a. The platinum electrode modified by the bromine compound is used as the working electrode, the mercurous sulfate electrode is used as the reference electrode, and the platinum electrode is used as the counter electrode, and the working electrode, the reference electrode and the counter electrode constitute a three-electrode system;

[0045] b. Put the three-electrode system into a solution prepared by 0.048g acrylamide, 0.036g N, N-methyleneisoacrylamide and 0.015g hemoglobin molecule, and apply a constant voltage between the working electrode and the reference electrode. Potential -0.8V, applied for 120min;

[0046] c. Put the working electrode into the eluent composed of 10% (v / v) acetic acid and 10% (v / v) sodium lauryl sulfate solution, soak for 120min, and elute hemoglobin molecules to obtain hemoglobin im...

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Abstract

The invention discloses a preparation method for hemoglobin molecularly imprinted polymers through autocatalysis of ATRP of a self-needed transitional metal catalyst. The method comprises the following steps: using a bromine-contained compound modified electrode as a working electrode, and allowing the working electrode with a reference electrode and a counter electrode to constitute a three-electrode system; placing the three-electrode system into a solution prepared from a functional monomer, a crosslinking agent and hemoglobin molecules, and applying a constant electric potential of minus 0.3 to minus 0.8 V for 60 to 240 min between the working electrode and the reference electrode so as to obtain hemoglobin polymers; and eluating hemoglobin molecules with an eluent so as to obtain the hemoglobin molecularly imprinted polymers.

Description

technical field [0001] The invention relates to a preparation method of a hemoglobin imprinted polymer, in particular to a method for preparing a hemoglobin imprinted polymer through self-catalyzed ATRP without a transition metal catalyst. Background technique [0002] Hemoglobin is an important protein responsible for carrying oxygen in higher organisms, and it participates in the CO 2 Clinically, the detection of hemoglobin can provide diagnostic basis for cardiovascular diseases, lung diseases, certain tumor diseases, etc. Therefore, sensitive and accurate detection of hemoglobin content in serum is of great significance. [0003] At present, the commonly used detection methods for hemoglobin include radioimmunoassay, enzyme-linked immunoassay, and molecular imprinting technology. Radioimmunoassay and enzyme-linked immunoassay need to prepare antibodies, and use the specific reaction between hemoglobin and its antibodies (ie, immune reaction) to test. The antibody prepa...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F222/38C08J9/26B01J20/26B01J20/30
Inventor 孙越杜洪莹兰玉廷冯春梁
Owner 内蒙古澳菲利食品股份有限公司
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