Paratyphoid B and Salmonella Javan Variation Kit, Preparation and Application

Abstract

The invention relates to an identification method for Salmonella paratyphi B and Salmonella paratyphi B javavar in the genus Salmonella, and mainly solves the technical problem of rapid screening and identification of Salmonella paratyphi B in clinical diarrhea cases, environment and food samples. The technical solution is: Salmonella paratyphi B and Salmonella paratyphi B Java variant identification kit, which is characterized by: including 1), Salmonella group B immune rabbit antiserum: containing Salmonella O: 4, O: 5 Diluted serum of factor antibody; 2), Salmonella phase H immunized rabbit antiserum: diluted serum containing Salmonella H phase b and 2 factor antibody respectively; 3), Potassium tartrate biochemical identification tube: peptone 10.0g, potassium sodium tartrate 10.0 g, sodium chloride 5.0g, phenol red 0.024g, agar 15.0g, distilled water 1000.0ml; 4), sterilized mineral oil; 5), H-phase induction soft agar: tryptone peptone 10.0g, casein 5.0g , Potassium nitrate 1.0g, agar powder 2.0g, distilled water 1000.0ml; 6), H phase b factor induced serum: contains induced serum H:b factor.

Description

technical field [0001] The invention relates to an identification method of Salmonella paratyphi B and Salmonella paratyphi B Java variety in Salmonella genus, that is, a kit, a preparation method and a use method for identification by combining serum diagnosis with biochemical reaction. Background technique [0002] Salmonella is an important food-borne infectious pathogen, which widely exists in farmed animals and the environment. There are more than 2,000 serotypes. Most of the Salmonella of subgenus I that are related to human infection need to be diagnosed by serotyping. Some serotypes have the same antigenic formula. Such as: Salmonella paratyphi b (4,5,12:b: 1,2 ) and Salmonella paratyphi B javavar (4,5,12:b: 1,2 ) both have the same H-phase antigen, so they cannot be identified solely by serological methods. The "Law of the People's Republic of China on the Prevention and Control of Infectious Diseases" has different classifications of biological hazards and publi...

AI Technical Summary

Problems solved by technology

For a long time, clinical laboratories and public health laboratories have been unable to identify and accurately report the identification results due to the similarity of the two antigens, or the correct reporting time has been delayed due to the induction test of the antigen, which directly affects the national infectious disease reporting system. Accurate case data and epidemic assessment of paratyphoid B

Foreign professional laboratories usually use molecular biology methods (PCR) to detect the d-potassium tartrate regulator (dt gene, dt positive for Java variant, dt negative for paratyphoid B), but this technique requires molecular The configuration requirements of diagnostic laboratories have obstacles to the popularization of applications for most domestic grassroots laboratories