Kit for salmonella paratyphi B and salmonella paratyphi B var Java, as well as preparation and using method of kit

Abstract

The invention relates to an identification method for salmonella paratyphi B and salmonella paratyphi B var Java in salmonella species, and mainly solves the technical problems of rapid screening and identification of salmonella paratyphi B from clinical diarrhea cases and environment and food samples. According to the technical scheme, a kit for identifying salmonella paratyphi B and salmonella paratyphi B var Java is adopted. The kit is characterized by comprising the following raw materials: (1) a salmonella B group immune rabbit anti-serum which contains diluted serums of salmonella O:4 and O:5 factor antibodies; (2) a salmonella H-phase immune rabbit anti-serum which contains diluted serums of salmonella H:b and H:2 factor antibodies; (3) a potassium tartrate biochemical identification tube which contains 10.0 g of peptone, 10.0 g of sodium potassium tartrate, 5.0 g of sodium chloride, 0.024 g of phenol red, 15.0 g of agar and 1000.0 ml of distilled water; (4) sterilizing mineral oil; (5) H-phase dedicated inducing soft agar which contains 10.0 g of tryptic soy agar, 5.0 g of casein, 1.0 g of potassium nitrate, 2.0 g of agar powder and 1000.0 ml of distilled water; (6) an H:b factor inducing serum which contains inducing serum H:b factors.

Description

technical field [0001] The invention relates to an identification method of Salmonella paratyphi B and Salmonella paratyphi B Java variety in Salmonella genus, that is, a kit, a preparation method and a use method for identification by combining serum diagnosis with biochemical reaction. Background technique [0002] Salmonella is an important food-borne infectious pathogen, which widely exists in farmed animals and the environment. There are more than 2,000 serotypes. Most of the Salmonella of subgenus I that are related to human infection need to be diagnosed by serotyping. Some serotypes have the same antigenic formula. Such as: Salmonella paratyphi b (4,5,12:b: 1,2 ) and Salmonella paratyphi B javavar (4,5,12:b: 1,2 ) both have the same H-phase antigen, so they cannot be identified solely by serological methods. The "Law of the People's Republic of China on the Prevention and Control of Infectious Diseases" has different classifications of biological hazards and publi...

AI Technical Summary

Problems solved by technology

For a long time, clinical laboratories and public health laboratories have been unable to identify and accurately report the identification results due to the similarity of the two antigens, or the correct reporting time has been delayed due to the induction test of the antigen, which directly affects the national infectious disease reporting system. Accurate case data and epidemic assessment of paratyphoid B

Foreign professional laboratories usually use molecular biology methods (PCR) to detect the d-potassium tartrate regulator (dt gene, dt positive for Java variant, dt negative for paratyphoid B), but this technique requires molecular The configuration requirements of diagnostic laboratories have obstacles to the popularization of applications for most domestic grassroots laboratories