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Method for preparing gamma-decalactone

A technology of decanolactone and lactone, applied in the field of bioengineering, can solve problems such as high cost and difficult separation, and achieve the effects of increasing production, increasing cumulative concentration and production, and maintaining activity

Active Publication Date: 2015-10-28
APPLE FLAVOR & FRAGRANCE GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above technical problems in the prior art, the present invention provides a method for preparing γ-decalactone, which solves the problem of high cost of the method in the prior art, separation difficult technical issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for yeast biotransformation of ricinoleic acid to prepare gamma-decalactone, comprising the following steps: yeast CICC32859 is made into a seed liquid, then inoculated into a transformation medium containing L-carnitine, and ricinoleic acid is used as Substrate, carry out aerobic biotransformation in shake flasks or fermenters, the temperature is controlled at 25-35°C, after transformation and cultivation for 36-48 hours, add γ-lactone 0.01-0.5g / L, continue to cultivate for 12-24H, biotransformation is completed, and a fermentation broth containing γ-decalactone is obtained; after the cultivation, a certain volume of fermentation broth is taken, extracted with three times the volume of n-hexane, and the organic phase is taken for gas phase detection. The content of gamma-decalactone in the fermentation broth was obtained, and gamma-decalactone was obtained by this method in the following examples.

[0018] The formula of the transformation medium is: ricinole...

Embodiment 2

[0020] Example 2 Comparative example

[0021] The yeast CICC32859 was inoculated in the slant medium for activation culture, and two rings of bacteria were picked from the activated slant with an inoculation loop and inoculated into a 250mL Erlenmeyer flask containing 50mL of seed medium, placed in a constant temperature shaker at 28°C, Cultivate at 180r / min for 20H to obtain mature seed liquid; transfer 10% inoculum by volume to a 250mL Erlenmeyer flask filled with 50mL transformation medium, and place in a constant temperature shaker at 30°C for transformation culture at 200r / min 84H, the biotransformation is over, and the fermentation broth containing γ-decalactone is obtained; take 2mL of the fermentation broth containing γ-decalactone, extract it with 6mL of n-hexane, take the organic phase for gas phase detection, and measure the The content of γ-decalactone is the highest and is 3.5g / L, the content of γ-decalactone in the fermentation broth at 72H is 3.1g / L, and the con...

Embodiment 3

[0023] Example 3 Effect of the addition of L-carnitine on the conversion of γ-decalactone

[0024] The yeast CICC32859 was inoculated in the slant medium for activation culture, and the tricyclic bacteria were picked from the activated slant with an inoculation loop and inoculated into a 250mL Erlenmeyer flask containing 50mL of seed medium, and placed in a constant temperature shaker at 28°C. Cultivate at 230r / min for 22H to obtain mature seed solution; transfer the seed solution to a 250mL Erlenmeyer flask containing 50mL of transformation medium at a 12% inoculum by volume, and place it in a constant temperature shaker at 32°C at 250r / min. After 84 hours of transformation and cultivation for 84 hours, the biotransformation was completed, and the fermentation broth containing γ-decalactone was obtained; 2 mL of the fermentation broth containing γ-decalactone was extracted with 6 mL of n-hexane, and the organic phase was taken for gas phase detection; The concentration of L-c...

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Abstract

The invention discloses a method for preparing gamma-decalactone. The method comprises the steps of preparing seed culture fluid with yeast CICC 32859 serving as bacteria, then inoculating the seed culture fluid to a transform culture medium, adding L-carnitine into the transform culture medium, adding a small quantity of gamma-decalactone compound into fermentation liquor in the transformation process, conducting biotransformation with ricinoleic acid serving as substrate, and preparing the gamma-decalactone. According to the method for preparing the gamma-decalactone, the product formation rate is promoted, the fermentation period is shortened, and more than that, a new method for restraining saccharomycetes to conduct open circle degradation on the product, namely the gamma-decalactone, is provided, so that the one-time cumulant of the gamma-decalactone is greatly increased.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a gamma-decalactone, specifically a method for preparing gamma-decalactone. Background technique [0002] γ-decalactone (gamma decalactone), also known as gamma decalactone, is an important aromatic compound containing a five-membered lactone ring. It has a pleasant peach and coconut aroma and has been widely used in the perfume industry. At present, there are three ways to obtain γ-decalactone: natural extraction, chemical synthesis and biological preparation. γ-decalactone naturally exists in peaches, almonds, strawberries and other fruits, so γ-decalactone can be extracted from fruits, but its content in fruits is very small, and the economic benefit is low. γ-decalactone can also be obtained by chemical synthesis, but the synthesized γ-decalactone is mainly a non-optical racemate, and there is no advantage in the optically active γ-decalactone extracted from fruits. And can't ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/04
Inventor 荣绍丰杨树林李茜茜蔡宝国管世敏王敬文周月
Owner APPLE FLAVOR & FRAGRANCE GRP
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