Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus mycoides strain and application thereof

The technology of Bacillus mycoides and strains, which is applied in the field of biological control preparations, can solve the problems of long breeding period of disease-resistant varieties, environmental pollution, single resistance, etc., and achieves the effects of good application prospect, wide action spectrum and strong adaptability.

Active Publication Date: 2015-10-28
山东颖秀环境科技有限公司
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of disease-resistant varieties is long and the resistance is single. However, chemical control can easily cause pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus mycoides strain and application thereof
  • Bacillus mycoides strain and application thereof
  • Bacillus mycoides strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Isolation and purification of Bacillus mycoides strain WWM09

[0028] Bacillus mycoides bacterial strain WWM09 of the present invention is to adopt dilution plate method and plate marking method to separate and obtain from seawater, and separation method is:

[0029] (1) Isolation of Bacillus: Water samples were collected at different locations in the Bohai Sea area in the northern part of Weifang, Shandong Province. Random sampling was used to collect appropriate amounts of seawater within 2.5 meters from the coast and mix well. Indicate the location, time and person of the collection. Weigh 1 g of water in 100 mL of sterile water, place in a shaker at 30°C at 150 rpm for 10 min, then place in a water bath at 60°C for 30 min, take 100 μL of 10 -1 、10 -2 、10 -3 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are pick...

Embodiment 2

[0050] 1. Fermentation process of Bacillus mycoides strain WWM09

[0051] The formula of LB liquid medium is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0052] A large amount of solid fermentation medium formula (mass percentage): solid material: mass ratio of 65% cornmeal, 20% soybean meal and 15% bran; inorganic salt solution: potassium dihydrogen phosphate 2.7%, magnesium sulfate 0.04%, ammonium sulfate 3.5%, the rest is water; the solid-liquid ratio is 1:2.0 (mass ratio).

[0053] Mass solid fermentation process of Bacillus mycoides strain WWM09:

[0054] ①Cultivation of strains in seed solution: Pick a small amount of bacteria from the slant of the test tube of Bacillus mycoides strain WWM09, transfer them to LB liquid medium, shake and culture at 30°C for 3-5 days, and this is the seed solution;

[0055] ②Cultivation of solid production strains: Inoculate the seed liquid into solid medium (500mL Erlenmeyer fla...

Embodiment 3

[0058] Example 3 field control test: 800 million / mL Bacillus mycoides WWM09 water agent prevents cucumber powdery mildew field efficacy 1. Test purpose

[0059] In order to verify the effect of the 800 million / mL Bacillus mycoides WWM09 water agent produced by this strain on the control effect of cucumber powdery mildew, this experiment was carried out.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacillus mycoides strain and application thereof, particularly the bacillus mycoides strain WWM09 with a preservation number of CGMCC NO.11047. The invention further discloses a biological control preparation containing the bacillus mycoides strain WWM09 and a preparation method of the biological control preparation. Experiments show that the bacillus mycoides strain WWM09 is large in diameter, resistant to hypoxia, wide in action spectrum and high in adaptability, and can be rapidly grown and multiplied in the low-nutrition state, thereby having a good application prospect; the biological control preparation prepared from the bacillus mycoides strain WWM09 can serve as biological pesticide or bio-fertilizer to control cucumber powdery mildew, grape downy mildew, rose powdery mildew and other leaf diseases, and further to control cotton damping-off, watermelon blight and other soil-borne plant diseases.

Description

technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a bacillus capable of efficiently preventing and controlling plant diseases and its use, and a biological control agent prepared by using the bacillus. Background technique [0002] Plant disease is a phenomenon in which plants undergo a series of morphological, physiological and biochemical pathological changes under the influence of biological or abiotic factors, hindering the normal growth and development process, thereby affecting human economic benefits. At present, for the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties and biological control. The breeding cycle of disease-resistant varieties is long and the resistance is single. Chemical control can easily cause pesticide residues and environmental pollution. At the same time, the l...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 牛赡光赵炳先李鹏李泉涌张淑静
Owner 山东颖秀环境科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com