IgG binding epitope of major soybean allergen gly m Bd 28K

A technology of major allergens and binding sites, applied in the fields of bioinformatics, immunology, molecular biology, and can solve problems such as elevation

Inactive Publication Date: 2018-06-22
HENAN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

[0006] In the soybean allergen protein, except for the IgG binding epitope of Gly m Bd 30K protein localized by Hosoyama H et al. using mouse monoclonal antibody, the IgG binding epitope of other soybean allergens has not been reported so far. In the detection of allergen-specific IgG antibodies, serum soybean allergen-specific IgG antibodies were significantly increased in patients with allergic diseases. Gly m Bd28K is the second most allergenic protein in soybean after Gly m Bd 30K, suggesting that allergen protein 28K is also IgG binding epitope present

Method used

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  • IgG binding epitope of major soybean allergen gly m Bd 28K
  • IgG binding epitope of major soybean allergen gly m Bd 28K
  • IgG binding epitope of major soybean allergen gly m Bd 28K

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Embodiment Construction

[0031] 1. Preparation of Gly m Bd 28K recombinant protein

[0032] Expression of 1 Gly m Bd 28K recombinant protein

[0033] Based on the known Gly m Bd 28K sequence in the NCBI Genbank gene bank (accession number: 21619.2), primers were designed for PCR amplification. The PCR product was detected and recovered, and connected with PMDT-19 to construct the cloning vector 28K-T. The 28K-T and pET-28a plasmids were digested with EcoRI and XhoI, respectively, to construct the recombinant plasmid pET-28K, which was transformed into Escherichia coli, and the expression of Gly m Bd 28K recombinant protein was induced by IPTG.

[0034] 2 Western blot detection

[0035] After IPTG-induced pET-28K whole bacteria and uninduced whole bacteria were subjected to 12% SDS-PAGE, the proteins separated by SDS-PAGE electrophoresis were charged and transferred to PVDF western blot membrane, blocked with 5% skimmed milk powder at 37°C for 1 hour, and washed with Tris The buffer was fully washed...

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Abstract

The invention relates to three IgG binding epitopes of a main soybean allergen Gly m Bd 28K. Amino acid sequences of the three IgG binding epitopes are 28H-Gly-Asp-Lys-Lys-Ser-Pro-Lys-Ser-Leu-Phe-Leu-Met-Ser-Asn-Ser-OH42, 56H-Leu-Lys-Ser-His-Gly-Gly-Arg-Ile-Phe-Tyr-Arg-His-Met-His-Ile-OH70 and 154H-Glu-Thr-Phe-Gln-Ser-Phe-Tyr-Ile-Gly-Gly-Gly-Ala-Asn-Ser-His-OH168 respectively. According to the IgG binding epitopes, suppression of the binding of a Gly m Bd 28K natural protein and a Gly m Bd 28K monoclonal antibody (mAb3F5) by the chemically synthesized polypeptide 56H-Leu-Lys-Ser-His-Gly-Gly-Arg-Ile-Phe-Tyr-Arg-His-Met-His-Ile-OH70 shows that the linear epitope polypeptide has a good suppression effect on affiliation of the Gly m Bd 28K natural protein and the Gly m Bd 28K monoclonal antibody (mAb3F5). The IgG binding epitopes are significant for proving a molecular mechanism produced by food allergy, understanding the effect of the allergen in food allergy, developing researches on non-allergic and hypoallergenic food and researching the occurrence of allergic diseases.

Description

technical field [0001] The invention relates to the fields of molecular biology, immunology and bioinformatics, in particular to the IgG binding epitope of soybean main allergen Gly m Bd28K protein. Background technique [0002] As one of the main plant protein resources, soybean protein is rich in amino acids necessary for the human body, does not contain cholesterol, and has high nutritional value, but a small number of people cannot benefit from it because they are allergic to soybeans and its products , the usual symptoms are rash, itchy skin, and diarrhea. Surveys have shown that about 1% to 6% of infants are affected by soybean allergy, and with the increase in consumption of soybean products, the incidence of soybean allergy in adults continues to rise. [0003] Allergen epitopes are the immunological material basis for triggering food allergic reactions. Epitopes are usually located on the surface of molecules, the size is equivalent to the binding site of antibodie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415
CPCC07K14/415
Inventor 席俊闫慧丽贺梦雪陆启玉
Owner HENAN UNIVERSITY OF TECHNOLOGY
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