Kit for detecting mycoplasma hominis nucleic acid through PCR-fluorescence probe method and detecting method of kit
A technology of mycoplasma hominis and fluorescent probe, which is applied in the field of medical detection and human virus detection, can solve the problems of lack of negative, positive control, high detection sensitivity, and insufficient specificity, so as to reduce false positive results, accurate detection results, The direct effect of identification results
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[0083] In order to describe the technical content of the present invention more clearly, further description will be given below in conjunction with specific embodiments.
[0084] One, the main components of the PCR-fluorescent probe method detection Mycoplasma hominis nucleic acid kit are shown in Table 1:
[0085] Table 1
[0086]
[0087] Prepare your own sterilized saline (0.9% NaCl), absolute ethanol, and 1.5ml EP tubes, and components from different batches cannot be mixed.
[0088] The nucleic acid kit for detection of Mycoplasma hominis by PCR-fluorescence probe method comprises: nucleic acid extraction solution, MH PCR amplification solution, MH positive control and negative control.
[0089] (1) Lysis solution: 5 mol / liter (M) guanidine hydrochloride, 0.5M potassium chloride, 10 mmol / liter (mM) tris(Tris), 1mM ethylenediaminetetraacetic acid (EDTA), 0.1% Triton X-100 (volume ratio), pH 6.4 at 25°C.
[0090] Proteinase K: 0.02 grams per liter (g / L), 1mM Tris, 1m...
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