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Liposome influenza virus antigen vaccine and preparing method thereof

A technology for influenza virus and antigen vaccines, applied in liposome delivery, antiviral agents, pharmaceutical formulations, etc., can solve the difficulties of epitope vaccines in inducing multiple immune responses, clinical application limitations, and difficulty in eliciting strong immune responses, etc. problems, to achieve stable performance, long shelf life, and reduce usage

Inactive Publication Date: 2015-10-21
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a liposomal influenza virus antigen for the defects of epitope vaccines in the prior art that it is difficult to induce a variety of immune responses, and it is difficult to cause a strong immune response and the clinical application is limited. Vaccine and its preparation method

Method used

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  • Liposome influenza virus antigen vaccine and preparing method thereof
  • Liposome influenza virus antigen vaccine and preparing method thereof
  • Liposome influenza virus antigen vaccine and preparing method thereof

Examples

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preparation example Construction

[0037] (1) preparation of influenza virus antigen vaccine stock solution;

[0038] Take any one or more strains of influenza virus, inoculate them on the virus culture medium respectively, culture them at 33°C-35°C for 48-72 hours, extract the influenza virus body, inactivate it with formaldehyde, concentrate it by ultrafiltration, Nano collider lyses the virus and purifies it by column chromatography to make the stock solution of influenza virus antigen vaccine;

[0039] Among them, the influenza virus strains include the three most common influenza virus strains, Type A (H1N1), Type A (H3N2) and Type B, which makes the stock solution of influenza virus antigen vaccine more practical;

[0040] (2) Preparation of empty liposomes;

[0041] Phospholipids, cholesterol and antioxidants are mixed and dissolved in an organic solvent, and after vacuum rotary evaporation, membrane-shaped empty liposomes are obtained;

[0042] Wherein, phospholipids include one or more of soybean lec...

Embodiment 1

[0054] (1) Preparation of influenza virus antigen vaccine stock solution

[0055] Get three kinds of influenza virus strains (comprising type A 1, type A 3 and type B), three kinds of influenza viruses are inoculated on the virus culture medium respectively, preferably, 5-10 days old healthy chicken embryo is used as virus culture medium; After culturing at 33°C-35°C for 48-72 hours, harvest allantoic fluid virus, slowly add 0.15%-4% formaldehyde at 36°C and let stand for 24 hours, then stand at room temperature for 24 hours to complete inactivation; Then in a 100KD ultrafiltration centrifuge tube, under the centrifugation condition of 3000g (g is the acceleration of gravity), carry out ultrafiltration concentration, nano collider cracking virus and purification by Sephadex G-200 (Sephadex G-200) column chromatography Make trivalent virus subunit influenza virus antigen vaccine stock solution;

[0056] Each mL of influenza virus antigen vaccine stock solution contains the fol...

Embodiment 2

[0073] Compared with Example 1, except that the preparation process of empty liposomes is different, other preparation processes are the same.

[0074] In the present embodiment, the preparation process of empty liposome is:

[0075] Mix and dissolve 400mg soybean lecithin for injection, 150mg cholesterol, and 50mg vitamin E in 100ml chloroform, put the liquid in an eggplant-shaped bottle of a rotary evaporator, use a water bath at 60°C as the evaporation temperature, and vacuum rotary evaporation to obtain Membrane empty liposomes.

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Abstract

The invention relates to a liposome influenza virus antigen vaccine and a preparing method thereof. The liposome influenza virus antigen vaccine is prepared with a film evaporation method. The preparing method includes the steps of preparing an influenza virus antigen vaccine stock solution, preparing empty liposome, synthesizing liposome and the like. Compared with a linear epitope vaccine, the liposome influenza virus antigen vaccine prepared with the preparing method of the liposome influenza virus antigen vaccine has the advantages that multiple or multiple kinds of antigens can be carried at the same time, various cellular immune responses can be induced, and high immune responses can be caused; in addition, the liposome influenza virus antigen vaccine is stable in performance and long in storage life, and meanwhile the use immunogen dosage can be reduced.

Description

technical field [0001] The invention relates to the field of influenza virus antigen vaccines, in particular to a liposome influenza virus antigen vaccine and a preparation method thereof. Background technique [0002] In the prior art, the epitope vaccines used to treat influenza are mainly chemically synthesized. Since the epitope only represents a part of the protein antigen, it is difficult to induce a variety of immune responses like the protein antigen vaccine; and the epitope vaccine It is usually linear and does not have the three-dimensional conformation of the antigenic epitope on the antigenic protein. Therefore, direct immunization with epitope peptides is difficult to elicit a strong immune response. Although a good immune response can be induced in a short period of time by linking the epitope peptide to the carrier molecule, it is difficult to guarantee the specificity of the immune response, and in order to achieve a good immune effect, on the one hand, the ...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K39/145A61K39/295A61P31/16
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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