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Method for improving hybridization success rate of sweet potato and incompatible material

A technology with high success rate and affinity, which is applied in the field of hybrid cultivation of sweet potatoes and incompatible materials, can solve problems such as heavy workload, chromosome loss, and low seed setting rate, and achieve strong practicability, high operability, and improved success rate effect

Inactive Publication Date: 2015-10-21
SAAS BIOTECH & NUCLEAR TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reasons are as follows: (1) Most of the natural hybrids between the parents are not fruiting, and the number of seeds is small; (2) Most wild sweet potato materials have complex ploidy, and the natural hybridization rate is low, even if the somatic cells are obtained by protoplast fusion technology Hybrids, also often cause a large number of chromosome loss in hybrids, hybrid sterility
(3) Sweet potato cultivars lacking broad hybridization compatibility
(4) The flowering period is not met, the workload is heavy, the seed setting rate is low, and the hybrid abortion
(5) Lack of systematic and complete incompatibility hybridization technology
This is also the main limiting factor that many existing methods cannot be successfully used in sweet potato breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Sweet potato hybrid varieties: female parent DY-3; male parent I. Trifida (6X).

[0023] (1) Material preparation: The sweet potato variety DY-3 was selected as the female parent and planted in the experimental ground temperature shed in spring and March. The male parent is I.Trifida(6X), sow its live seeds in the wild resource ground, apply enough base fertilizer, and set up a frame to cultivate it to make it bloom naturally; the flowering period of the male parent I.Trifida(6X) is from June to October , the budding time of the female parent variety DY-3 is in early July.

[0024] (2) Hybridization: When the female parent variety DY-3 enters the peak flowering period, the flower buds that will bloom the next day are selected, detasselled and bagged. At 8-9 am the next day, pollen from I. Trifida (6X) was collected and given to the stigma of emasculated sweet potato DY-3 pistils, bagged and marked.

[0025] (3) Plant growth regulator treatment: 24 hours after pollinat...

Embodiment 2

[0030] Sweet potato hybrid varieties: female parent DY-3; male parent I. Trifida (6X).

[0031] (1) Material preparation: The sweet potato variety DY-3 was selected as the female parent and planted in the greenhouse of the test site in spring and March. The male parent is I.Trifida(6X), sow its live seeds in the wild resource ground, apply enough base fertilizer, and set up a frame to cultivate it to make it bloom naturally; the flowering period of the male parent I.Trifida(6X) is from June to October , the budding time of the female parent variety DY-3 is in early July.

[0032] (2) Hybridization: When the female parent variety DY-3 enters the peak flowering period, the flower buds that will bloom the next day are selected, detasselled and bagged. At 8-9 am the next day, pollen from I. Trifida (6X) was collected and given to the stigma of emasculated sweet potato DY-3 pistils, bagged and marked.

[0033] (3) Plant growth regulator treatment: 28 hours after pollination, dip ...

Embodiment 3

[0038] Sweet potato hybrid varieties: female parent DY-3; male parent I. Trifida (6X) close wild species.

[0039] (1) Material preparation: The sweet potato variety DY-3 was selected as the female parent and planted in the greenhouse of the test site in spring and March. The male parent is I. Trifida (6X), sow its live seeds in the wild resource ground, apply enough base fertilizer, and set up a frame to cultivate it to make it bloom naturally; the flowering period of the male parent I. Trifida (6X) close wild species is In August, the budding time of the female parent variety DY-3 is in August.

[0040] (2) Hybridization: When the female parent variety DY-3 enters the peak flowering period, the flower buds that will bloom the next day are selected, detasselled and bagged. At 8-9 am the next day, pollen from I. Trifida (6X) was collected and given to the stigma of emasculated sweet potato DY-3 pistils, bagged and marked.

[0041] (3) Plant growth regulator treatment: 28 hou...

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Abstract

The invention discloses a method for improving the hybridization success rate of sweet potato and an incompatible material. The method comprises the following steps: performing material preparation, hybridization treatment and plant growth regulator treatment; smearing the growth regulator on female parent stigmas and ovaries thereof after pollination, then continuing cultivation, and performing young embryo rescue and seedling cultivation. The method disclosed by the invention is higher in operability and high in practicability, the success rate of sweet potato inter-species incompatible hybridization can be obviously improved, and the method is also suitable for sweet potato intra-species incompatible hybridization.

Description

technical field [0001] The invention relates to a crop hybrid breeding method, in particular to a hybrid cultivation of sweet potato and incompatible materials. Background technique [0002] Sweet potato (Ipomoea batatas L.) is an important food crop and economic crop in the world. Variety improvement is an important means to increase yield, quality and resistance. However, at present, a large number of bred varieties are used as parents in sweet potato breeding, which will lead to narrow genetic background of new bred varieties, reduced hybrid vigor, and less and less room for improving yield, quality and resistance in the future. Wild sweet potato related wild species have excellent traits such as disease resistance, high dryness rate, and stress resistance, and have large genetic differences from sweet potato. Using efficient hybridization technology, the excellent genes of sweet potato related wild species are introduced into sweet potato, and sweet potato is created. N...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00
Inventor 冯俊彦蒲志刚李明张聪阎文昭屈会娟谭文芳杨松涛王大一
Owner SAAS BIOTECH & NUCLEAR TECH RES INST
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