Artificial fruiting method for black fungus
A kind of black fungus, artificial technology, applied in the fields of botanical equipment and methods, horticulture, plant cultivation, etc., can solve the problems of large demand for forest resources, can not be promoted, difficult to mass-produce black fungus, etc., to ensure nutrition and purity. Effects of taste, energy saving and labor
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Embodiment 1
[0026] A kind of black fungus artificial ear method, comprises the steps:
[0027] (1) Preparation of wild black fungus virus-free pure strains: collect black fungus fruiting body and open it after disinfection with 70% alcohol solution under aseptic conditions, and take 0.2-0.6mm of it 3 Fresh bacteria meat tissue on the surface is inoculated in sterile corn debris medium, and cultured in the dark at room temperature for 30 days, the mycelium is covered with the surface of the solid medium, and then the mycelia with strong mycelium growth and white and dense mycelium are taken. The mycelium is expanded and cultivated, that is, the virus-free pure strain of the wild black fungus is obtained;
[0028] (2) Making pure strains of wheat grains: take unbroken, plump wheat, boil in water for 20 minutes, remove, dry the surface moisture to obtain dried wheat, mix in 0.3% of the mass of the dried wheat Plaster of Paris powder, sealed, sterilized at 100°C for 150 minutes and then cool...
Embodiment 2
[0034] A kind of black fungus artificial ear method is characterized in that, comprises the steps:
[0035] (1) Preparation of wild virus-free pure strains of wild black fungus: From April 10 to May 10, at a temperature of 15°C, in the wild tree growth area in the upper reaches of the Qinggeli River Valley in Qinghe County, select gray-black, shape-like cascades Wild black fungus fruiting bodies with fan shape and thickness ≥ 2mm are collected, and the collected black fungus fruiting bodies are transported to the aseptic inoculation room with a fresh-keeping box, and the black fungus fruiting bodies are collected from being transported to aseptic inoculation Indoor time ≤ 6h, to guarantee the freshness of black fungus fruiting body; Then the black fungus fruiting body in described aseptic inoculation room is placed on the ultra-clean workbench, open sterile wind blowing 30min; Use 75% alcohol to supernatant The surface of the fruiting body of the black fungus on the clean work...
Embodiment 3
[0042] A kind of black fungus artificial ear method is characterized in that, comprises the steps:
[0043] (1) Preparation of wild black fungus virus-free pure strains: when the temperature is 15°C, select wild black fungus fruiting bodies with grayish black color, shape such as stacked fans, and thickness ≥ 2mm for collection, and collect the collected black fungus The fruiting body is transported to the aseptic inoculation room with a fresh-keeping box, and the time for the black fungus fruiting body to be transported to the aseptic inoculation room from collection is less than or equal to 6 hours to ensure the freshness of the black fungus fruiting body; then the aseptic inoculation room The black fungus fruiting body is placed on the ultra-clean workbench, and the aseptic wind is blown for 45 minutes; the surface of the black fungus fruiting body on the ultra-clean workbench is sterilized with an alcohol mass fraction of 90% to ensure the preparation There is no interfere...
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