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Microbacterium capable of completely degrading zearalenone and application thereof

A gibberellone, completely degradable technology, applied in the field of microorganisms

Inactive Publication Date: 2015-09-30
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that strains of the genus Microbacterium can degrade xanthan gum, chitosan, chloroacetyl and a variety of compounds containing benzene rings, but there is no report on the strains of the genus Microbacterium degrading erythralenone

Method used

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  • Microbacterium capable of completely degrading zearalenone and application thereof
  • Microbacterium capable of completely degrading zearalenone and application thereof
  • Microbacterium capable of completely degrading zearalenone and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Screening, isolation and identification of zearalenone-degrading strains

[0021] Soil samples were collected from turf (N36.34.243 E100.32.285) near Qinghai Lake on the Qinghai-Tibet Plateau. Take 2 g of soil samples, add 10 mL of 0.15M sodium chloride aqueous solution, shake for 30 min to fully disperse the samples, let stand at room temperature for 1 min, immediately take 1 mL of supernatant, spread on LB plates, and incubate at 30 °C until single For clonal growth, single colonies of different shapes were picked and inoculated in liquid LB medium (adding zearalenone with a final concentration of 25 μg / ml dissolved in methanol), cultured at 30°C for 2 days, centrifuged to obtain the supernatant, and washed with an appropriate amount of ethyl acetate. Extract, collect ethyl acetate and evaporate to dryness, dissolve the eluate with a small amount of methanol, and use TLC method (petroleum ether: acetone: ethyl acetate = 5:4:1 as the developing solvent) to...

Embodiment 2

[0023] Example 2: Microbacterium sp. Fermentation of FY1538

[0024] Microbacterium sp. The fermentation supernatant of FY1538 strain is used to degrade erythralenone, which needs to be induced by methanol. After isolation and purification of the strains, a single colony was picked and inoculated in 50 mL of LB medium, and methanol at a final concentration of 10 μl / ml was added, shaken and cultured at 30 °C for 48 h, and the fermentation broth was centrifuged at 12,000 rpm to obtain the fermentation supernatant.

Embodiment 3

[0025] Example 3: Microbacterium sp. Analysis of the ability of FY1538 fermentation supernatant to degrade erythralenone

[0026]Take 500μl of the fermentation supernatant, add 25ug zearalenone, react in a water bath at 30°C for 24 hours, extract the reaction solution with 1000μl ethyl acetate, collect the ethyl acetate phase, evaporate to dryness and dissolve the eluate with a small amount of methanol, use TLC method ( MERCK company TLC silica gel plate 60F 254 , the developer is petroleum ether: acetone: ethyl acetate = 5:4:1) to check the residue of erythralenone. Such as image 3 as shown, Microbacterium sp. After 24 hours of catalytic reaction between FY1538 fermentation supernatant and zearalenone, no zearalenone in the reaction system could be detected by TLC method.

[0027] Further analysis by HPLC Microbacterium sp. The ability of FY1538 fermentation supernatant to degrade erythralenone at different reaction times. The HPLC chromatographic column was Agile...

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Abstract

The invention belongs to the technical field of microorganisms and particularly relates to a microbacterium capable of completely degrading zearalenone and application thereof. The microbacterium capable of completely degrading zearalenone, discovered by the invention is named Microbacterium sp. FY1538. The Microbacterium sp. FY1538 belongs to Microbacterium sp. and is separated from turf on the bank of the Qinghai Lake of the Qinghai-Tibet plateau. Microbacterium sp. FY1538 is cultured in an LB culture medium with a pH value of 7.4 at a culture temperature of 30 DEG C for 48h in a shaking way by virtue of a shake flask at a rotating speed of 220rpm in the presence of methyl alcohol with a final concentration of 10 mu l / ml; the fermentation supernate has the capability of completely degrading zearalenone. The microbacterium provided by the invention can be applied to detoxification for mouldy crops such as mouldy corn.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a microbacterium and its application for completely degrading erythralenone. Background technique [0002] Zearalenone (ZEN), also known as F-2 toxin, is a mycotoxin with estrogen-like biological activity, mainly produced by Fusarium ( Fusariumsp .) Certain species of fungi, such as Fusarium graminearum ( Fusarium graminearum ) etc. are produced through the polyketide anabolic pathway. It can lead to human or animal reproductive system disorders, clinical manifestations of hyperestrogenism, and potential carcinogenicity and liver and kidney damage. Fusarium has strong ecological adaptability and is widely distributed in both parasitic and saprophytic life. The physical and chemical properties of erythralenone are stable, insoluble in water, and difficult to remove. In many links of crop growth, harvesting, storage, and processing, it is very easy to be att...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015A23K1/00C12R1/01
Inventor 吕红徐天宇周峻岗余垚胡苏莹
Owner FUDAN UNIV
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