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Hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and polysaccharide component on-line quantitative analysis method thereof

A liquid chromatography and extraction device technology, which is applied to the field of experimental devices for sample pretreatment, can solve unseen and other problems, and achieve the effects of simple structure, good result reproducibility, and improved purification effect.

Active Publication Date: 2015-09-23
丽水智客汇信息服务有限公司
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Problems solved by technology

After searching, there are no patent disclosures similar to the present invention, and bibliographical reports on quantitative analysis of biological samples carried out with similar experimental devices of the present invention

Method used

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  • Hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and polysaccharide component on-line quantitative analysis method thereof
  • Hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and polysaccharide component on-line quantitative analysis method thereof
  • Hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and polysaccharide component on-line quantitative analysis method thereof

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Embodiment Construction

[0036] A detailed description will be given below of specific embodiments of the present invention.

[0037] Such as figure 1As shown, a hollow fiber membrane liquid-phase micro-extraction liquid chromatography device, the device includes a sampling part 2, a liquid chromatography pump 4, a six-way valve 5, a chromatographic column 6, a detector 7, a sample loop 9 , a waste liquid bottle 10 and an ultrasonic cleaning machine 11, a hollow fiber membrane liquid-phase microextraction device 1 is arranged in the described ultrasonic cleaning machine 11, and a liquid chromatography universal Pipeline 8, one end liquid chromatography common pipeline 8 is connected with described six-way valve 5 and sampling part 2, and described six-way valve 5 is connected with described liquid chromatography pump 4, chromatographic column 6 respectively , the sample loop 9 is connected to the waste liquid bottle 10, the chromatographic column 6 is connected to the detector 7, and the detector 7 i...

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Abstract

The invention relates to a hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device and a polysaccharide component on-line quantitative analysis method thereof. The device comprises a sample feeding part, a liquid chromatography pump, a six-way valve, a chromatographic column, a detector, a sample ring, a waste liquid bottle and an ultrasonic washer, wherein a hollow-fiber membrane liquid-phase micro-extraction device is arranged inside the ultrasonic washer; general liquid chromatography pipelines are arranged at two ends of the hollow-fiber membrane liquid-phase micro-extraction device; the general liquid chromatography pipeline at one end is connected to the six-way valve and the sample feeding; the six-way valve is respectively connected to the liquid chromatography pump, the chromatographic column, the sample ring and the waste liquid bottle; the chromatographic column is connected to the detector; the detector is connected to the waste liquid bottle. The hollow-fiber membrane liquid-phase micro-extraction and liquid chromatography coupling device has the characteristics as follows: samples are efficiently and quickly purified; the method is scientific and reasonable; the extraction conditions are adjustable; the application prospect is good.

Description

technical field [0001] The invention relates to an experimental device for sample pretreatment, in particular to a hollow fiber membrane liquid-phase micro-extraction liquid-phase chromatography device and an online quantitative analysis method for polysaccharide components. Background technique [0002] Polysaccharides are natural polymer compounds that widely exist in nature and are composed of monosaccharides linked by glycosidic bonds. The determination of monosaccharide composition of polysaccharides is the most important link to control the quality standards of polysaccharides and provide basic information of polysaccharides. Monosaccharide composition is a basic and important content of sugar analysis. Since most sugars do not have optical properties, when the sugars are separated and determined by chromatography or electrophoresis, ultraviolet or fluorescent reagents are often used to derivatize the sugars to improve their optical activity and detection sensitivity....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王娜妮寿旦费莹
Owner 丽水智客汇信息服务有限公司
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