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Preparation and detection method of tetracycline aptamer sensor based on screen-printed electrode

A tetracycline aptamer and screen-printed electrode technology, applied in the direction of material electrochemical variables, can solve the problems of weak immune sensor signal, unsatisfactory reproducibility, and poor reusability, so as to avoid cross-interference and small sample amount , to achieve the effect of commercialization

Inactive Publication Date: 2015-09-23
SHANDONG UNIV OF TECH
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  • Abstract
  • Description
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Problems solved by technology

Antibiotics are small molecule hapten substances, and it is difficult to prepare corresponding antibodies. Even if corresponding antibodies are prepared, there are still problems such as weak signal of immune sensor, poor reusability, and reproducibility that cannot meet the requirements of actual sample detection.

Method used

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  • Preparation and detection method of tetracycline aptamer sensor based on screen-printed electrode
  • Preparation and detection method of tetracycline aptamer sensor based on screen-printed electrode
  • Preparation and detection method of tetracycline aptamer sensor based on screen-printed electrode

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Embodiment

[0018] Embodiment: (1) The preparation method of the ionic liquid is as follows: 4mg N-octylpyridinium hexafluorophosphate (ionic liquid) is dispersed in 4mL of absolute ethanol, ultrasonically dispersed for 6 hours to obtain a stable dispersion, The obtained highly dispersed suspension was a 1 mg / mL ionic liquid solution, and the prepared ionic liquid solution was stored at 4 °C. (2) The preparation method of the described ferric oxide is as follows: take 1mL acetic acid solution in a small beaker, add 100mL ultrapure water to make 1.0% acetic acid solution. Add 0.1g chitosan to 50mL acetic acid solution to make 0.2% chitosan solution. Take 4mL of 0.2% chitosan solution, add 1mg of ferric oxide powder, and ultrasonically disperse for more than 6h to obtain a stable dispersion. (3) Cleaning of the screen-printed electrode: cleaning and activating the screen-printed carbon electrode, the process is: first, place the screen-printed carbon electrode in NaOH solution for ultrason...

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Abstract

The invention relates to a preparation and detection method of a tetracycline aptamer sensor based on a screen-printed electrode. The surface of the screen-printed electrode is modified with an ionic liquid and ferroferric oxide layer by layer and then left to stand at a normal temperature until the surface is dried, and then tetracycline aptamer is fixed on the surface of the screen-printed electrode modified with a nanometer material. The preparation and detection method of the tetracycline aptamer sensor based on the screen-printed electrode well solves the problem about complicated procedures of current electrode pre-treatment, the same batch of electrodes have good uniformity and can be produced in batch, and commercialization is realized. The sensor has better sensitivity, stability and specificity and good regeneration capacity and is very suitable for situations when on-site quick detection of antibiotic residues is required; the recovery rate of the sensor meets requirements.

Description

technical field [0001] The invention relates to a method for preparing and detecting an aptamer sensor based on a screen-printed electrode, and belongs to the technical field of agricultural product safety detection. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, milk production has increased significantly, and fresh milk and dairy products have become an important part of people's (especially the elderly and children's) daily food. Antibiotics are widely used to prevent and treat dairy cow diseases. Due to frequent and overdose use, there are a certain amount of antibiotic residues in milk and other animal source foods. Drinking or eating milk or dairy products with antibiotic residues for a long time is equivalent to long-term low-dose antibiotic intake, which will inhibit the normal flora in the human intestinal tract and cause a large number of pathogenic bacteria to cause systemic or local infection. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
Inventor 郭业民孙霞焦艳翠王相友
Owner SHANDONG UNIV OF TECH
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