Kit for detecting child active tuberculosis and application of kit

Abstract

The invention discloses a kit for detecting child active tuberculosis and an application of the kit. The kit is combined with a bioinformatics method to screen characteristic metabolic products of child active tuberculosis, and a detecting method for the child active tuberculosis is established based on chemical shift of the characteristic metabolic products of child active tuberculosis; experiments show that in verification of 24 samples by the detecting method provided by the invention, the accuracy is 100%, the specificity is 100% and the sensitivity is 100%; in addition, the detecting method is simple and easy to operate and can be used for early detection of child active tuberculosis; the detecting method provides a new approach for clinical diagnosis of child active tuberculosis as well as provides new thoughts for exploring disease mechanisms.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for detecting active tuberculosis in children and its application. Background technique [0002] Tuberculosis caused by Mycobacterium tuberculosis (MTB) is one of the most common human infectious diseases in the world today and is considered to be the second largest disease "killer" after AIDS. Therefore, tuberculosis has become a threat to human health. The most important disease is a difficult problem of global public health control. China is one of the 22 countries with a high incidence of tuberculosis in the world, and the number of tuberculosis patients ranks second in the world. The epidemic situation of tuberculosis among children in my country is also severe. The fourth national tuberculosis epidemiological sampling survey report in 2000 showed that the infection rate of tuberculosis among children aged 0-14 in my country was 9.0%, and the prevalence of ac...

AI Technical Summary

Problems solved by technology

[0003] However, for a long time, the diagnosis of tuberculosis in children has faced two practical problems that are difficult to solve: first, the bacterial load of tuberculosis patients in children is low, coupled with poor cough reflex and tracheal ciliary motility in children, often accompanied by extrapulmonary tuberculosis, etiological diagnosis Positive rate is very low

When the clinical symptoms of children with tuberculosis are not obvious in the early stage, timely and accurate etiological results cannot be obtained, and the diagnosis is often delayed, resulting in the deterioration of the disease and the loss of the best time for rescue

2. When the immune function is low, or the child suffers from severe tuberculosis such as systemic miliary tuberculosis and tuberculous meningitis, or the child has used immunosuppressants, the immune response of the body is weakened, and the routine immunological diagnosis method often has false negatives, which seriously affects the diagnosis of the disease. Significant adverse effects of diagnosis and early preventive treatment

This diagnostic technology overcomes a series of deficiencies in the traditional medical diagnostic model, including low diagnostic accuracy, single diagnostic index, strong dependence on expert diagnosis and treatment, and lack of individualized treatment models. , make a more accurate and objective evaluation, and avoid misdiagnosis of human factors

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