A polysaccharide protein complex of willow enoki mushroom and its preparation method and application

A technology of Flammulina velutipes polysaccharides and complexes, which can be used in drug combination, food science, allergic diseases, etc. It can solve the problems of extraction and biological activity of Flammulina velutipes polysaccharides without willow, and achieve important economic value and market value.

Active Publication Date: 2019-02-26
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no products related to the proteoglycan of Flammulina velutipes in the domestic and foreign markets, and there are no reports on the extraction and biological activity of polysaccharides from Flammulina velutipes in the literature

Method used

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  • A polysaccharide protein complex of willow enoki mushroom and its preparation method and application
  • A polysaccharide protein complex of willow enoki mushroom and its preparation method and application
  • A polysaccharide protein complex of willow enoki mushroom and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed culture medium, the culture conditions are: temperature 26°C, rotation speed 120r / min, after cultivating for 5 days, insert the fermentation medium with 10% inoculation amount, the fermentation conditions are: temperature 26°C, rotation speed 120r / min, after culturing for 5 days, centrifuge at 4000r / min to collect the supernatant to obtain a crude polysaccharide-protein complex fermentation broth; the composition of the fermented seed medium of Flammulina velutipes polysaccharides is: 200g of potatoes; 15g of glucose; KH 2 PO 4 1.5g; MgSO 4 ·7H 2 O, 0.75g; V B1 , 1mg; water, 1000mL; fermentation medium composition: glucose, 100g; corn flour 200g; yeast extract powder, 60g; KH 2 PO 4 , 1.5g; MgSO 4 ·7H 2 O, 7.5g; V B1 , 10mg; water, 1000mL;

[0016] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation liquid by rotary evaporation to 1 / 10 of the original body, add 3 t...

Embodiment 2

[0018] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed culture medium, the culture conditions are: temperature 24°C, rotation speed 140r / min, after cultivating for 5.5 days, insert the fermentation culture medium with 15% inoculum amount, the fermentation conditions are: The temperature is 24°C, the rotational speed is 140r / min, and after culturing for 5 days, the supernatant is collected by centrifugation at 4000r / min to obtain a crude polysaccharide-protein complex fermentation broth; the composition of the willow flammulina velutipes polysaccharide fermentation seed medium is: potato 250g; glucose 17.5g; KH 2 PO 4 1.75g; MgSO 4 ·7H 2 O, 0.88g; V B1 , 1.25mg; water, 1000mL; fermentation medium composition: glucose, 150g; corn flour 300g; yeast extract powder, 60g; KH 2 PO 4 , 1.75g; MgSO 4 ·7H 2 O, 8.8g; V B1 , 12.5mg; Water, 1000mL;

[0019] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation broth to 1 / 9 of the ori...

Embodiment 3

[0021] (1) Fermentation: Inoculate Flammulina velutipes strains in the seed medium, the culture conditions are: temperature 22°C, rotation speed 160r / min, after cultivating for 6 days, insert the fermentation medium with 20% inoculum, the fermentation conditions are: temperature 22°C, rotation speed 160r / min, after culturing for 6 days, centrifuge at 6000r / min to collect the supernatant to obtain the crude polysaccharide-protein complex fermentation broth; the composition of the willow flammulina velutipes polysaccharide fermentation seed medium is: 300g of potatoes; 20g of glucose; KH 2 PO 4 2.0g; MgSO 4 ·7H 2 O, 1g; V B1 , 1.5mg; water, 1000mL; fermentation medium composition: glucose, 200g; corn flour 400g; yeast extract powder, 60g; KH 2 PO 4 , 2.0g; MgSO 4 ·7H 2 O, 10g; V B1 , 15mg; water, 1000mL;

[0022] (2) Extraction: Concentrate the crude polysaccharide-protein complex fermentation broth to 1 / 8 of the original body by rotary evaporation, add 5 times the volum...

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PUM

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Abstract

The invention relates to a polysaccharide-protein complex of Flammulina velutipes and its preparation method and application, and belongs to the field of natural medicine development. Inoculate the strain of Flammulina velutipes in the seed culture medium. The culture conditions are: temperature 22-26°C, rotation speed 120-160r / min. After 5-6 days of cultivation, 10-20% of the inoculation amount is inserted into the fermentation medium. The fermentation conditions It is: temperature 22-26°C, rotation speed 120-160r / min, after culturing for 5-6 days, centrifuge at 4000r / min-6000r / min to collect the supernatant, and concentrate it to 1 / 10-1 / 8 of the original volume by rotary evaporation. Add 3-5 times the volume of absolute ethanol to precipitate at 4°C for 12-16h, centrifuge for 10-20min at a speed of 3000-5000r / min, collect the precipitate, and dry at 45-50°C. The invention fills up the gap in the development and utilization of Flammulina velutipes resources in the market. At the same time, it provides an important foundation for the in-depth development of polysaccharide immunostimulant drugs and health food with immunoregulatory effects, and has important economic and market value.

Description

technical field [0001] The invention belongs to the field of natural medicine development, and in particular relates to a fermented liquid extract of Flammulina velutipes and its preparation method and application. Background technique [0002] Edible and medicinal fungi are a very important class of natural biological resources, and have become an important research object in the discovery of new drugs and the development of functional foods. Studies have shown that edible and medicinal fungi contain polysaccharides, proteins, flavonoids and other active substances, which have various pharmacological effects such as improving immunity, anti-tumor, and anti-oxidation. At present, Lentinan, Ganoderma lucidum polysaccharide, and Polyporus polysaccharide have been approved by the health department and have become clinical anti-tumor immunotherapy drugs. Although a large number of edible and medicinal fungus polysaccharides have been developed and utilized, due to the rich fung...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/06A61P37/02A61P35/00A23L33/10
Inventor 王琦朱宴妍刘洋魏巍朱相杨王瑞
Owner JILIN AGRICULTURAL UNIV
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