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A kind of assay method of betaine in wolfberry

A determination method and betaine technology, applied in the field of detection and analysis, can solve the problems of not meeting the needs of sample detection, affecting the results of quantitative analysis, not suitable for betaine determination, etc., to avoid the use of toxic and harmful organic solvents, high-sensitivity determination, Satisfy the detection effect

Inactive Publication Date: 2016-05-25
JINAN INST OF FRUIT PRODS CHINA GENERAL SUPPLY & MARKETING COOP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two standards both use ion chromatography, but they are not suitable for the determination of betaine in samples with high sugar content, and there is interference of sodium ions in the determination process, which seriously affects the quantitative analysis results
In addition, other standards involving the determination of betaine have complex operations and high detection limits, such as NY / T1746-2009 "Determination of Betaine in Beetroot", the detection limit is 400mg / kg, which is far from meeting the actual sample detection needs

Method used

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  • A kind of assay method of betaine in wolfberry
  • A kind of assay method of betaine in wolfberry
  • A kind of assay method of betaine in wolfberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Selection of chromatographic conditions for ion chromatographic analysis

[0029] (1) Selection of chromatographic column

[0030] The experiments compared the commonly used IonPacCS17 chromatographic column and IonPacCS19 chromatographic column. The results showed that when the IonPacCS17 column was used for analysis, the chromatographic peaks of other impurities in the wolfberry samples could not be completely separated from the betaine chromatographic peaks; using the IonPacCS19 chromatographic column, the separation effect was significantly better than that of the IonPacCS17, and the retention time of betaine was stable within 123 days. The relative standard deviation of retention times was less than 1.1%.

[0031] (2) Column temperature and analysis time conditions

[0032] When the temperature is maintained at 30 °C, the best column efficiency is obtained, and the separation from adjacent impurity peaks is complete, with high separation degree and good...

Embodiment 2

[0034] Embodiment 2 Determination experiment of betaine in Lycium barbarum sample

[0035] The method was applied to the determination of betaine in wolfberry samples.

[0036] (1) Sample pretreatment

[0037] Weigh about 0.5g of the crushed solid sample (the solid sample is wolfberry fruit) or about 2.5g of the liquid sample, accurately weigh, add water to dissolve and dilute to 25ml, ultrasonically treat (500W / 40KHz) for 30min, centrifuge for 10min (rotating speed 10000rpm) ), after taking the supernatant filter membrane, pre-activating the RP column, discarding the first 3 mL, taking the subsequent filtrate, and running it on the machine for testing.

[0038] (2) Configuration of standard solution

[0039] Betaine standard stock solution 1000mg / L: Accurately weigh 0.1000g of standard substance and dissolve in pure water, shake well for use. Then dilute to the required concentration step by step. Standard substance chromatogram (chromatographic conditions are the same as...

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Abstract

The invention discloses a determination method for glycine betaine in wolfberry fruits. The method comprises the following steps: (1) extracting wolfberry fruit samples with a water extracting solvent, and taking a filtering solution obtained by filtering as a solution to be detected; and (2) analyzing and determining glycine betaine in the samples by combining an ion chromatography with a pulse ampere detector. The invention establishes the method for determining glycine betaine in the wolfberry fruits by combining the ion chromatography with the pulse ampere detector. The method has good linearity; and the recycling rate is 90.8%-94.7%, the accuracy is 1.11% and the detection limit is 0.0366 micrograms / milliliter. According to the method, a toxic reagent is not used; and a test verifies that the method is applicable to rapid and batch detection of glycine betaine in various phytophagous and functional foods.

Description

technical field [0001] The invention relates to an analysis method for measuring betaine in wolfberry, and belongs to the technical field of detection and analysis. Background technique [0002] Lycium barbarum (medlar) is the dry fruit of the Solanaceae and perennial deciduous shrubs. Modern pharmacological research generally believes that the active ingredients and trace elements of wolfberry have pharmacological effects. There are three main types of active ingredients in wolfberry, and betaine is one of them. Betaine is a class of quaternary ammonium salt compounds with molecular formula C 5 H 11 NO 2, Structural formula (CH 3 ) 3 N + -CH 2 -COO - , the relative molecular weight is 117.15. It is one of the main alkaloids in the fruit, stalk and leaves of Lycium barbarum, and it acts as a methyl donor in plants. At present, the research on the determination method of betaine content is increasing day by day, mainly including titration method, colorimetric metho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 闫新焕宋烨朱风涛刘雪梅郑晓冬孟晓萌潘少香李仁勇
Owner JINAN INST OF FRUIT PRODS CHINA GENERAL SUPPLY & MARKETING COOP
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