Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for establishing mouse model with hepatic preneoplastic lesion caused by double factors of alcohol and HBV

A technology of precancerous lesions and mouse models, which is applied to the active ingredients of hydroxyl compounds, animal husbandry, etc., to achieve the effect of easy repeatability, low cost and simple production

Active Publication Date: 2015-09-16
GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no recognized model of liver cancer induced by alcoholism and concurrent hepatitis virus infection for experimental research. How to choose and study a more ideal model of liver cancer precancerous lesions is the key

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for establishing mouse model with hepatic preneoplastic lesion caused by double factors of alcohol and HBV
  • Method for establishing mouse model with hepatic preneoplastic lesion caused by double factors of alcohol and HBV
  • Method for establishing mouse model with hepatic preneoplastic lesion caused by double factors of alcohol and HBV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] 1. Materials

[0028] 1.1 Animals 1.3 copies of high-replication HBV transgenic mice, male, 6-8 weeks old, weighing 23 ± 2g, 32 were purchased from the Army Liver Disease Center of the 458th Hospital of the Chinese People's Liberation Army, animal qualification certificate number: SCXK (military ) 2012-0018.

[0029] 1.2 The alcohol content of the reagent is 53% vol Red Star Erguotou Liquor, produced by (Beijing Red Star Co., Ltd.), product standard number: GB / T10781.2; 4% paraformaldehyde (Biotoopped Company).

[0030] 1.3 Instruments TB-215D electronic balance (Denver, USA); YB-6LF biological tissue paraffin embedding machine (Hunan Yaguang Company); RM2235 tissue slicer (Germany LEICA); YT-7F slicer (Hunan Yaguang Company); BX51 microscope (Japan OLYMPUS Company).

[0031] 2. Method

[0032] 2.1 Preparation of liver cancer precancerous lesion mouse model After adaptive feeding of 32 HBV transgenic mice for one week, they were randomly divided into 16-week control ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for establishing a mouse model with a hepatic preneoplastic lesion caused by double factors of alcohol and the HBV. The method includes the following steps that firstly, according to an animal, a high-duplication HBV transgenic mouse is copied in the substep of 1.3; secondly, white spirit serves as the reagent; thirdly, the mouse model with the hepatic preneoplastic lesion is prepared, the HBV transgenic mouse is fed with the white spirit every day, continuous gavage is conducted for 16-30 weeks, and a mouse eats and drinks freely during the period; fourthly, after-treatment of the model mouse with the hepatic preneoplastic lesion is conducted, wherein after gavage, weighing is conducted respectively, the mouse is taken and put to death, hepatic tissue is taken out, the hepar is weighed, the hepar index is calculated, and pathological section observation is conducted. The method has the advantages that the animal model is easy to manufacture, low in cost and easy to repeat; the HBV transgenic mouse model induced by alcohol for 22 weeks can be put under a scope so that it can be seen that atypical hyperplasia has large-cell and small-cell changes, the method conforms to the criteria of the hepatic preneoplastic lesion model, and successful model making is displayed and deduced.

Description

field of invention [0001] The invention relates to the field of animal models, in particular to a method for establishing a mouse model of liver cancer precancerous lesions caused by ethanol and HBV. Background technique [0002] Liver cancer is a malignant tumor of the liver, which can be divided into two categories: primary and secondary. Primary malignant tumors of the liver originate from the epithelial or mesenchymal tissues of the liver. The former is called primary liver cancer, which is a malignant tumor with a high incidence in my country and is extremely harmful; rare. Secondary or metastatic liver cancer refers to malignant tumors originating from multiple organs throughout the body and invading the liver. Generally, it is more common in liver metastases of malignant tumors in stomach, biliary tract, pancreas, colorectum, ovary, uterus, lung, breast and other organs. [0003] There are about 350 million people in the world who are chronic carriers of hepatitis B...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/027A61K31/045
CPCA01K67/0276A01K2207/25A01K2217/30A61K31/045
Inventor 唐东昕杨柱郭斌
Owner GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com