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A method for increasing the saponin content in the adventitious roots of American ginseng cultured in a reactor by using ginseng pathogenic elicitors

A technology for ginseng pathogenic bacteria and ginseng rust rot bacteria, which is applied in the biological field, can solve the problems of high price, short supply and the like, and achieves the effects of wide applicability, precise and accurate detection, and increased production of saponins

Active Publication Date: 2017-07-28
廉美兰
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  • Application Information

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Problems solved by technology

Due to the great medical and health care uses of American ginseng, the demand is increasing year by year, resulting in a situation in which the supply exceeds demand and the price is expensive

Method used

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  • A method for increasing the saponin content in the adventitious roots of American ginseng cultured in a reactor by using ginseng pathogenic elicitors
  • A method for increasing the saponin content in the adventitious roots of American ginseng cultured in a reactor by using ginseng pathogenic elicitors
  • A method for increasing the saponin content in the adventitious roots of American ginseng cultured in a reactor by using ginseng pathogenic elicitors

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Embodiment Construction

[0020] (1) Materials and methods

[0021] ① Fungal culture

[0022] The ginseng black spot fungus and the ginseng rust fungus were respectively inoculated into petri dishes containing PDA medium, and cultivated at 25°C. After 20-25 days, the hyphae were scraped and inoculated in PDA liquid medium for liquid shaking culture, and the culture period was 15-20 days.

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Abstract

The invention relates to a method for increasing the content of saponin in the adventitious root of American ginseng cultivated in a reactor by using the elicitor of ginseng pathogenic bacteria. When the adventitious roots of American ginseng were cultivated in the reactor for 30 days and the biomass reached the maximum, 4mg / L black spot elicitor was added to treat the adventitious roots of American ginseng. The yield was also the highest, at 273.7mg / L. The invention can effectively increase the content of saponins in adventitious roots, combined with the means of tissue culture, can obtain a large amount of American ginseng adventitious roots with high ginsenoside content in a short period of time as a substitute medicinal material for American ginseng, which provides a resource utilization and protection for the precious Chinese herbal medicine American ginseng A convenient and effective method.

Description

technical field [0001] The invention relates to the field of biotechnology, and relates to a method for increasing the content of saponins in adventitious roots of American ginseng by using elicitors of ginseng pathogenic bacteria. Background technique [0002] American ginseng (Panax quinquefolium L.) is also known as American ginseng, American ginseng, American ginseng, American ginseng, and Cantonese ginseng. Native to the eastern United States and Canada, it is a perennial herbaceous plant of the Araliaceae genus Panax, whose roots are used as medicine. American ginseng has many medical effects such as nourishing, strengthening the heart, hematopoiesis, invigorating the stomach, calming, lowering blood fat, anti-cancer and anti-aging. Modern chemical and pharmacological studies have shown that the main active ingredient of American ginseng is saponin. Compared with ginseng, its nature is milder, and the content of total saponins and ginsenoside Rb1 is higher than that ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N15/82A01G1/00
Inventor 廉美兰朴炫春李铁军廉哲雄
Owner 廉美兰
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