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Curcumin derivative preparing method

A technology of curcumin derivatives and curcumin, which is applied in the field of preparation of curcumin derivatives, and can solve the problems of poor absorption ability, curcumin's long-lasting efficacy, low selectivity, etc.

Active Publication Date: 2015-09-09
ZHONGRONG TECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of curcumin in the body, such as not lasting efficacy, low selectivity, poor ability to be absorbed by the body, easy to be degraded, and low bioavailability, its application in the field of health food and medicine is restricted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation of curcumin transformation product

[0039] (1) Slant culture: inoculate Rhodococcus ZJPH1003 into the slant medium, and cultivate at 30°C for 3 to 4 days to obtain slant strains; the final concentration of the slant medium consists of: glucose 10g / L, peptone 5.0g / L , yeast extract 3.0g / L, (NH 4 ) 2 SO 4 4.0g / L,KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, NaCl 0.5g / L, agar 30g / L, solvent is water, pH 7.0, sterilized at 121°C for 20 minutes, cooled after sterilization, and made into a slope;

[0040] (2) Seed culture: pick a ring of thalli from the slant of step (1) and inoculate it into a 250mL shake flask equipped with 100mL seed medium, cultivate at 30°C and 200rpm for 24h, and prepare a seed solution; the seed culture The base final concentration is composed of: glucose 10g / L, peptone 5.0g / L, yeast extract 3.0g / L, (NH 4 ) 2 SO 44.0g / L,KH 2 PO 4 1.0g / L, K 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O 0.5g / L, NaCl 0.5g / L, ...

Embodiment 2～7

[0066] The effect of embodiment 2~7 thalline concentration on the transformation preparation product 4 of Rhodococcus ZJPH1003

[0067] Utilize Rhodococcus sp. (Rhodococcus sp.) ZJPH1003 bacterial strain, after the method for fermenting and cultivating by embodiment 1 step (4), wet thallus is added in the 50mL Erlenmeyer flask that 10mL 0.1M, pH 6.6 phosphate buffer are housed (wet bacteria Body is respectively 14.4g / L, 21.6g / L, 28.9g / L, 36.1g / L, 43.3g / L, 50.5g / L) (shown in table 2) with thalline dry weight, substrate curcumin The final concentration was 50mg / L, and the transformation was carried out at 30°C and 200rpm for 36h. After the reaction, the transformed liquid was centrifuged to remove the bacteria to obtain a supernatant, which was extracted three times with ethyl acetate, combined with the extracts, concentrated to dryness at 50°C under reduced pressure, and the residue obtained after rotary evaporation was then used in 0.75ml The chromatographic methanol was re-d...

Embodiment 8～14

[0071] Examples 8-14 Effect of the pH value of the transformation solution on the transformation of Rhodococcus ZJPH1003 to produce product 4

[0072] Utilize Rhodococcus sp. (Rhodococcus sp.) ZJPH1003 bacterial strain, after fermenting and cultivating according to the method of step (4) of Example 1, add the wet thalline into 10mL 0.1M, pH is respectively 5.4~8.0 phosphate buffer (Table 3) Shown) in the 50mL Erlenmeyer flask (wet thalline final concentration is 43.3g / L), substrate curcumin concentration is 50mg / L, at 30 ℃, 200rpm transformation 36h. After the reaction was over, the cells were removed by centrifugation to obtain the supernatant, which was extracted three times with ethyl acetate, the extracts were combined, concentrated under reduced pressure to dryness to remove ethyl acetate, and the residue obtained after rotary evaporation was then washed with 0.75ml chromatographic methanol After re-dissolving, the detection method is the same as that in step (7) of Examp...

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Abstract

The invention discloses a curcumin derivative preparing method. The curcumin derivative preparing method includes the steps that a conversion reaction is conducted in a phosphate buffer solution with pH value of 5.4-8.0 under the conditions of 25-35 DEG C and 150-250 rpm with wet cells obtained through fermentation cultivation of rhodococcus ZJPH1003 as an enzyme source and curcumin as a substrate, conversion reaction liquid is subjected to postprocessing after the conversion reaction is ended, and curcumin derivatives are obtained. A rhodococcus ZJPH1003 bacterial strain resting cell conversion method is utilized for conducting structural modification on the curcumin, and then the corresponding curcumin derivatives are obtained. The pharmacological or biological activity of the modified curcumin derivatives is improved to different extents compared with the unmodified curcumin substrate, and development of new medicinal preparations is facilitated. The technological process of adopting the microbial conversion method to obtain the curcumin derivatives is simple and environmentally friendly; biocatalyst is made of microbial cells, can be fermented and prepared automatically and has a stable quality and low cost.

Description

(1) Technical field [0001] The invention relates to a preparation method of curcumin derivatives. (2) Background technology [0002] The chemical structural formula of curcumin is: [0003] [0004] Curcumin (curcumin) is a natural active ingredient extracted from the rhizomes of turmeric, curcuma, turmeric, etc., which have anti-cancer, anti-oxidation, anti-inflammatory, anti-HIV, anti-hypertension, anti-cholesterol, Anticoagulation and other biological activities, and its small molecular weight, low toxicity, has good potential for clinical application. However, due to the shortcomings of curcumin in the body, such as not lasting drug effect, low selectivity, poor ability to be absorbed by the body, easy to be degraded, and low bioavailability, its application in the field of health food and medicine is restricted. Therefore, on the basis of retaining the original efficacy of curcumin, the preparation of curcumin derivatives with better performance by chemical or biol...

Claims

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Application Information

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IPC IPC(8): C12P7/22C12P7/26C12R1/01
CPCC12P7/22C12P7/26
Inventor 王普黄金罗杨春
Owner ZHONGRONG TECH CORP LTD
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