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Dragonfly intestinal bacterium aspergillus terreus QT122 and metabolite and application thereof

A technology of QT122 and metabolites, which is applied to the intestinal symbiotic Aspergillus terreus strain and its metabolites and application fields, can solve the problems of chemical herbicide environmental pollution, pesticide residue weed resistance, etc., and achieve the effect of good herbicidal activity

Active Publication Date: 2015-09-09
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the wide application of chemical herbicides, its disadvantages are becoming more and more prominent. The problems of chemical herbicides causing environmental pollution, pesticide residues and weed resistance have attracted great attention of pesticide scientific and technological workers. Development of new efficient, safe and pollution-free weed control agent imminent

Method used

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  • Dragonfly intestinal bacterium aspergillus terreus QT122 and metabolite and application thereof
  • Dragonfly intestinal bacterium aspergillus terreus QT122 and metabolite and application thereof
  • Dragonfly intestinal bacterium aspergillus terreus QT122 and metabolite and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Isolation, purification and identification of dragonfly intestinal symbiotic Aspergillus terreus (Aspergillus terreus QT122):

[0032] The tested dragonflies were collected from the suburbs near Zhejiang Normal University. The captured dragonflies were starved for 24 hours, disinfected with 75% (volume %) alcohol for 2 minutes under aseptic conditions, rinsed with sterile water for 3 times, and dissected with sterile tweezers. Take out the intestinal tract and put it in a sterile mortar, add a small amount of sterile water for grinding, and then dilute it to 10% with sterile water -1 、10 -2 、10 -3 three concentration gradients. Take 0.2 mL of each concentration gradient dilution solution and spread it on the malt solid medium (MEA medium), and culture it upside down in a 28°C incubator. After the colony grows, pick a small amount of hyphae from the edge of the colony and transfer it to a new MEA medium. Repeat this transfer to obtain a single colony, and ...

Embodiment 2

[0038] Embodiment 2, the liquid fermentation of dragonfly intestinal symbiotic Aspergillus terreus (Aspergillus terreus QT122):

[0039] Activation of Aspergillus terreus (Aspergillus terreus QT122): Take the mycelium block (about 1-2g) with bacteria from the slope of the MEA test tube of the preserved strain, put it on the fresh malt solid medium (MEA), and put it in a 28°C incubator Inverted culture, the activated Aspergillus terreus (Aspergillus terreus QT122) was obtained.

[0040] Inoculate the mycelium block (about 2 to 3 g) of the activated Aspergillus terreus QT122 into a 250 mL Erlenmeyer flask containing 150 mL of malt liquid medium (ME medium), at 28 ° C, 170 rpm / min Cultivate under conditions for 3 days as seed solution; inoculate 6-7 bottles in total.

[0041] Then, according to the inoculation amount of 1% volume ratio, 5 ml of the seed solution was transferred into a 1000 mL Erlenmeyer flask equipped with 500 mL of malt liquid medium (ME medium), and cultivated...

Embodiment 3

[0043] Example 3. Separation and purification of metabolites from dragonfly intestinal symbiotic Aspergillus terreus QT122:

[0044] The 40L fermented liquid prepared according to the method described in Example 2 was filtered through 4 layers of gauze, and the obtained filtrate was extracted with an equal volume of ethyl acetate for a total of 3 times, and the extract was concentrated and dried in a vacuum (in 0.1 negative Pressed vacuum degree, 45 ℃ drying 30~40 minutes), obtain brown fermented broth crude extract (26.23g).

[0045] The obtained fermented broth crude extract (26.23g) was crudely separated by silica gel column chromatography (200-300 mesh silica gel, about 260g), and gradient elution was carried out using dichloromethane / methanol. The volume ratios are: 100:0, 100:1, 100:2, 100:4, 100:8, 100:16, 100:32; the amount of each eluent is about 5100ml, 4500ml, 3750ml, 3000ml, 2250ml, 1500ml, 1500ml, and the flow rate is 10ml / min . Different gradient elutions were ...

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Abstract

The invention discloses aspergillus terreus QT122 which is Aspergillus terreus QT122, and its preservation number is CCTCC M 2015240. The invention also discloses preparation methods of an aspergillus terreus QT122 fermentation broth, an aspergillus terreus QT122 fermentation broth crude extract and an aspergillus terreus QT122 metabolite. The invention also discloses an application of aspergillus terreus QT122. The aspergillus terreus QT122 fermentation broth, the aspergillus terreus QT122 fermentation broth crude extract and the aspergillus terreus QT122 metabolite all can be used for inhibiting radicle growth of amaranthus retroflexus or barnyard grass. The aspergillus terreus QT122 fermentation broth, the aspergillus terreus QT122 fermentation broth crude extract and the aspergillus terreus QT122 metabolite can be used in the preparation of a biopesticide with an inhibitory activity to radicle growth of amaranthus retroflexus and barnyard grass.

Description

technical field [0001] The invention belongs to the field of microbial technology agricultural production, and relates to a dragonfly intestinal symbiotic Aspergillus terreus QT122 strain, metabolites and applications thereof. Background technique [0002] Insects are the most diverse group of organisms known in the earth's biosphere, and the ad hoc microorganisms that live in symbiosis with insects are rich in diversity. According to existing research reports, we can know that insect symbiotic bacteria not only have a wide variety, but also have certain particularities in terms of ecology, metabolic characteristics, and physiological activities. Therefore, they may be a wide source of new drug source molecules such as antibacterial and herbicides. . However, compared with insect species, there are less studies on insect symbionts and their metabolites. Therefore, research on insect symbionts and their secondary metabolites is of great significance for the development and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04C07C50/18C07C49/597C07C69/92A01N45/02A01N35/06A01N37/10C12R1/66
Inventor 张应烙卢贻会
Owner ZHEJIANG NORMAL UNIVERSITY
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