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Method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria

A technology of synergy and compound strains, applied in the field of environmental bioengineering, to achieve the effects of improving governance effects, improving treatment efficiency, and increasing tolerance range

Inactive Publication Date: 2015-08-19
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for treating hexavalent chromium pollution by utilizing the synergistic effect of composite bacteria to address the shortcomings of prior art physical and chemical methods and single microorganisms to control hexavalent chromium pollution

Method used

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  • Method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria
  • Method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria
  • Method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Acclimatization screening steps:

[0034] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;

[0035] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;

[0036] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...

Embodiment 2

[0045] Acclimatization screening steps:

[0046] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;

[0047] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;

[0048] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...

Embodiment 3

[0057] Acclimatization screening steps:

[0058] (1) Take 10 g of chromium-contaminated soil sample and add it to 100 ml of beef extract-peptone liquid medium, and incubate at 30°C for 24 h with a shaker speed of 120 rpm;

[0059] (2) Take 1 ml of the culture solution in step (1) and transfer it into 100 ml of inorganic salt liquid medium. The medium contains 20 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. Shaker speed is 120 rpm;

[0060] (3) Take 1 ml of the culture solution in step (2) and transfer it to 100 ml of inorganic salt liquid medium. The medium contains 30 mg / L of hexavalent chromium, prepared with potassium dichromate, and cultured at 30°C for 24 hours. The rotation speed of the shaker was 120 rpm, and so on, and the rest of the conditions were kept constant. The content of hexavalent chromium was gradually increased in increments of 10 mg / L until the concentration of hexavalent chromium reached 50 mg / L. Aft...

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Abstract

The invention relates to a method for processing hexavalent chromium pollution employing synergistic effects of mixed bacteria, and belongs to the technical field of environment bioengineering. The processing method provided by the invention comprises the following steps: separating, purifying and screening a bacillus: B1 and a geotrichum: G1 from hexavalent chromium-polluted soil; individually cultivating the bacillus and the geotrichum to obtain a single bacterium solution; and inoculating the bacterium solution into a same polluted water body at the volume ratio of 1 to 1, and mixing and cultivating for processing hexavalent chromium pollution. The bacillus and the geotrichum are mixed and inoculated into chromium-containing wastewater; the defect that a single bacterium has strict requirements on inflow pH is overcome by different tolerance ranges of the pH values (pH) of two bacteria and the synergistic effects of the bacteria; the initial pH tolerance range of an inoculating microbe on the wastewater is improved; the preprocessing cost is reduced; and meanwhile, the processing effect on the hexavalent chromium-polluted wastewater is improved.

Description

technical field [0001] The invention relates to a method for treating heavy metal chromium pollution, in particular to a method for treating hexavalent chromium pollution by synergistic action of compound bacteria, and belongs to the technical field of environmental bioengineering. Background technique [0002] Chromium is widely used in electroplating, printing and dyeing, tanning, metallurgy and other industries. The pollution caused by improper treatment of chromium-containing wastewater and chromium slag is becoming more and more serious. Chromium exists mainly in the form of trivalent chromium and hexavalent chromium in nature. Chromium valence is not easily soluble in water, has strong binding force with soil and other media, has poor fluidity, and has low toxicity, while hexavalent chromium has strong mobility and high biological toxicity, which can cause distortion and cancer, and damage internal organs. Industrial pollutants that are harmful to the human body. [0...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C12N1/14C02F3/34C12R1/07C12R1/645C02F101/22
Inventor 许燕滨屈毛毛许诗慧李亚飞何晓琳
Owner GUANGDONG UNIV OF TECH
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