Apparatus and method for producing anti-Her-2 monoclonal antibodies through online industrially robust regulation of cell states

A cell state, industrially robust technology, applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve incomplete or only partial control, lack of precise adjustment of cell state And control, less control of expression process parameters, etc., to reduce the risk of bacterial contamination, maintain stability, and maintain growth vitality

Inactive Publication Date: 2015-08-19
上海泰因生物技术有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Although these recombinant protein production processes have been enlarged to more than 1000L, the process itself has the following disadvantages: 1) The parameters of the cultivation and expression process are less controlled, and the physical and chemical conditions of the culture environment such as temperature and pH cannot be fully or only partially controlled , CO 2 and O 2 concentration, nutrient concentration, etc.; 2) Even if the

Method used

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  • Apparatus and method for producing anti-Her-2 monoclonal antibodies through online industrially robust regulation of cell states
  • Apparatus and method for producing anti-Her-2 monoclonal antibodies through online industrially robust regulation of cell states
  • Apparatus and method for producing anti-Her-2 monoclonal antibodies through online industrially robust regulation of cell states

Examples

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example 1

[0082] Example 1. The device and method for the production of recombinant anti-Her-2 monoclonal antibody by online industrially robust (industrially robust) regulation and control of CHO cell state bioreactor

[0083] The reagents and instruments used in this example are as follows:

[0084] Culture medium: Excell CD CHO serum-free medium produced by Sigma, USA;

[0085] Cell: CHO cell stable strain expressing recombinant anti-Her-2 monoclonal antibody;

[0086] Monitorable cell bioreactor: 7L animal cell reactor produced by Applicon Company in the Netherlands;

[0087] Online monitoring system: the control tower produced by Applicon Company in the Netherlands.

[0088] The method for producing anti-Her-2 monoclonal antibody in this example is as follows:

[0089] 1) On the control tower produced by Applicon Company in the Netherlands, the temperature is set at 37°C, the pH value is 7.1, the dissolved oxygen concentration is 45%, and the dissolved carbon dioxide concentrati...

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Abstract

The present invention discloses a bioreactor cell culture apparatus capable of onlinely and continuously monitoring physiological and biochemical indexes in a variety of cell culture environments and directly regulating and controlling cell states (including cell cycle and the like), and a method for producing anti-Her-2 monoclonal antibodies through the apparatus. With the apparatus and the method of the present invention, the bacterial pollution risk during the cell culture process can be reduced, the stable cell growth environment can be maintained, the constant cell growth viability can be maintained, and the high-quality, high-efficiency and continuous anti-Her-2 monoclonal antibody production can be achieved.

Description

technical field [0001] The present invention relates to a device and method for producing anti-Her-2 monoclonal antibody by adjusting and controlling cell state on-line in the field of bioengineering, in particular to an on-line industrially robust continuous monitoring and continuous perfusion biological reaction The device and method for cell culture, expansion and preparation of anti-Her-2 monoclonal antibody can directly adjust and control the cell state on-line to adjust various physical and biochemical indicators in the cell culture environment of the device and the culture bottle / bag system. Background technique [0002] Breast cancer is a type of disease rather than a disease. Its occurrence involves the imbalance of apoptosis and proliferation, cell differentiation disorder, dysfunction of oncogenes and tumor suppressor genes, and abnormalities in various life activities such as related signal transduction systems. As an oncogene closely related to the metastasis an...

Claims

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Application Information

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IPC IPC(8): C12M1/36C12M1/34C12Q3/00C12P21/08
Inventor 齐念民
Owner 上海泰因生物技术有限公司
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