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Hyaluronic acid-chitosan microsphere carrying epidermal growth factor and preparation method and application of hyaluronic acid-chitosan microsphere

A technology of epidermal growth factor and chitosan microspheres, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, skin diseases, etc. It can protect the wound surface, improve the quality of healing, and speed up the healing speed.

Active Publication Date: 2015-08-19
SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the emulsification solvent evaporation method and the cross-linking polymerization method, organic solvents or cross-linking agents are required in the preparation, which will cause environmental pollution on the one hand, and on the other hand, there are organic solvents or cross-linking agent residues in the finished product
Although the spray drying method does not use organic solvents, the drying temperature is generally high, which is likely to cause denaturation of protein or polypeptide drugs

Method used

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  • Hyaluronic acid-chitosan microsphere carrying epidermal growth factor and preparation method and application of hyaluronic acid-chitosan microsphere
  • Hyaluronic acid-chitosan microsphere carrying epidermal growth factor and preparation method and application of hyaluronic acid-chitosan microsphere

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of hyaluronic acid-chitosan microspheres loaded with epidermal growth factor

[0036] (1) Hyaluronic acid (average relative molecular mass 0.12×10 6 ) 0.2g, sodium tripolyphosphate 0.05g, and epidermal growth factor 0.1g were dissolved in 1000ml of purified water.

[0037] (2) Chitosan (average relative molecular mass 0.08×10 6 , deacetylation degree 88%) 1.2g dissolved in 1000ml purified water.

[0038] (3) Add the solution obtained in step (1) to the solution obtained in step (2) and stir and mix continuously with an electromagnetic stirrer, the addition speed is 50ml / min, the addition time is 20min, the stirring speed is 300 rpm, and the stirring time is 30min , to obtain a suspension containing hyaluronic acid-chitosan microspheres.

[0039] (4) Centrifuge the suspension obtained in the above step (3) with a centrifugal force of 10000g and a centrifugation time of 30min; discard the supernatant and collect the precipitate.

[0040] (5) Add the precip...

Embodiment 2

[0044] Preparation of hyaluronic acid-chitosan microspheres loaded with epidermal growth factor

[0045] (1) Hyaluronic acid (average relative molecular mass: 0.06×10 6 ) 0.4g, sodium tripolyphosphate 0.1g, and epidermal growth factor 0.1g were dissolved in 1000ml of purified water.

[0046] (2) Chitosan (average relative molecular mass 0.08×10 6 , deacetylation degree 88%) 2g dissolved in 1000ml purified water.

[0047] (3) Add the solution obtained in step (1) to the solution obtained in step (2) and stir and mix continuously with an electromagnetic stirrer, the addition speed is 100ml / min, the addition time is 10min, the stirring speed is 600 rpm, and the stirring time is 30min , to obtain a suspension containing hyaluronic acid-chitosan microspheres.

[0048] (4) Centrifuge the suspension obtained in the above step (3) with a centrifugal force of 10000g and a centrifugation time of 30min; discard the supernatant and collect the precipitate.

[0049] (5) Add the precipita...

Embodiment 3

[0053] Preparation of Film Containing Epidermal Growth Factor Microspheres

[0054] A. Take 120g of polyvinyl alcohol (model 2699) and add it to 1000ml of purified water to dissolve, take 10g of soluble starch, add it and stir to dissolve. Heat the above solution to 70°C-80°C, add 20g of glycerin, 10g of Tween, and 8g of simethicone oil respectively and stir, leave it at room temperature to cool down, filter, add 2g of hyaluronic acid-chitosan microbe loaded with epidermal growth factor Ball, spare.

[0055] B. Prepare an aqueous solution of 10% polyvinyl alcohol for later use.

[0056] C. Prepare 4% nitrocellulose alcohol-ether mixture (alcohol-ether volume ratio 1:1) for later use.

[0057] D. Prepare 4% collagen in glacial acetic acid solution for later use.

[0058] Apply liquid B evenly on a glass plate, dry it in vacuum at 40°C, then apply liquid C, liquid A, and liquid D in sequence, dry it in vacuum at 40°C, and divide it into small pieces to obtain the product.

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Abstract

The invention provides an environment-friendly and simple preparation method for a hyaluronic acid-chitosan microsphere carrying an epidermal growth factor. The hyaluronic acid-chitosan microsphere is prepared via ionic polymerization, centrifugation, purification, and freeze-drying under the protection of a protective agent. The hyaluronic acid-chitosan microsphere carrying the epidermal growth factor can be used for preparing pharmaceutical preparations (including a gel, a film agent, a powder agent, an ointment, and the like) for treating burn and scald wounds. According to the hyaluronic acid-chitosan microsphere carrying the epidermal growth factor and the pharmaceutical preparations of the hyaluronic acid-chitosan microsphere, sustained release of the epidermal growth factor on the burn and scald wounds is realized; wound cell proliferation is promoted; and functions, of protecting burn and scald tissues and promoting wound healing, of sodium hyaluronate and chitosan are developed. The hyaluronic acid-chitosan microsphere can provide a support for wound cell proliferation, and an excellent medicine for treating burn and scald wounds is obtained.

Description

Technical field: [0001] The invention designs a hyaluronic acid-chitosan microsphere loaded with epidermal growth factor, a preparation method thereof and a pharmaceutical composition using the same as an active ingredient. Background technique: [0002] Burns are one of the most painful and difficult diseases of all diseases. At present, the treatment system that occupies a dominant position in the field of burn treatment is to promote the dry scab on the wound surface, and then perform local treatment of scab excision and skin grafting, combined with systemic treatment such as anti-shock, anti-infection, and nutritional support. This treatment method greatly reduces the mortality rate of extensive burns, but there are serious disadvantages such as great pain for patients, high incidence of scars, and high medical expenses. Therefore, it is of positive significance to find new treatments and drugs. In severe skin defects caused by burns and wound healing, it is very impor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K38/18A61K47/36A61P17/02
Inventor 凌沛学曹心珂金艳张岱州董爱梅刘正平张新房祝美华李大伟孙利民
Owner SHANDONG ACADEMY OF PHARMACEUTICAL SCIENCES
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